redFluor™ 710 Anti-CD45 antibody [RA3-6B2] (ab242264)
![Flow Cytometry - redFluor™ 710 Anti-CD45 antibody [RA3-6B2] (ab242264)](https://www.abcam.com/ps/products/242/ab242264/Images/ab242264-331189-anti-cd45-antibody-ra3-6b2-redfluor-710-flow-cytometry.jpg "Flow Cytometry - redFluor™ 710 Anti-CD45 antibody [RA3-6B2] (ab242264)")
Key features and details
- redFluor™ 710 Rat monoclonal [RA3-6B2] to CD45
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: redFluor™ 710
- Isotype: IgG2a
Overview
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Product name
redFluor™ 710 Anti-CD45 antibody [RA3-6B2]
See all CD45 primary antibodies -
Description
redFluor™ 710 Rat monoclonal [RA3-6B2] to CD45 -
Host species
Rat -
Conjugation
redFluor™ 710 -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: C57Bl/6 splenocytes.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
RA3-6B2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab242264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. 0.125 µg: The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. |
Target
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Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. -
Involvement in disease
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
Domain
The first PTPase domain interacts with SKAP1. -
Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 19264 Mouse
- SwissProt: P06800 Mouse
- Unigene: 391573 Mouse
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Alternative names
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
Images
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Flow Cytometry - redFluor™ 710 Anti-CD45 antibody [RA3-6B2] (ab242264)
Flow Cytometric analysis of C57Bl/6 bone marrow cells staining CD45 using 0.125 µg ab242264 (solid line) or 0.125 µg redFluor™ 710 Rat IgG2a isotype control (dashed line).
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![Flow Cytometry - redFluor™ 710 Anti-CD45 antibody [RA3-6B2] (ab242264)](https://www.abcam.com/ps/products/242/ab242264/Images/ab242264-331188-anti-cd45-antibody-ra3-6b2-redfluor-710-flow-cytometry.jpg)
Flow Cytometry - redFluor™ 710 Anti-CD45 antibody [RA3-6B2] (ab242264)Flow Cytometric analysis of C57Bl/6 bone marrow cells staining CD45 using 0.125 µg ab242264 (solid line, left panel) or 0.125 µg Alexa Fluor® 700 Anti-Hu/Mo CD45R (RA3-6B2) (solid line, right panel).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab242264 has not yet been referenced specifically in any publications.
