redFluor™ 710 Anti-CD45RA antibody [HI100] (ab218648)
![Flow Cytometry - redFluor™ 710 Anti-CD45RA antibody [HI100] (ab218648)](https://www.abcam.com/ps/products/218/ab218648/Images/ab218648-274364-anti-cd45ra-antibody-hi100-redfluor-710-flow-cytometry.jpg "Flow Cytometry - redFluor™ 710 Anti-CD45RA antibody [HI100] (ab218648)")
Key features and details
- redFluor™ 710 Mouse monoclonal [HI100] to CD45RA
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: redFluor™ 710
- Isotype: IgG2b
Overview
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Product name
redFluor™ 710 Anti-CD45RA antibody [HI100]
See all CD45RA primary antibodies -
Description
redFluor™ 710 Mouse monoclonal [HI100] to CD45RA -
Host species
Mouse -
Conjugation
redFluor™ 710 -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee
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Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- FC; Human peripheral blood lymphocytes.
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General notes
Conjugated to redFluor™ 710. RedFluor™ 710 dye is excited by the red (633-647 nm) laser and has a peak emission of 710 nm. The recommended band pass filter for this dye is 710/50. RedFluor™ 710 can be used as an alternative for Alexa Fluor® 700. Confirm that your cytometer is configured to detect this fluorochrome.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab218648 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
HI100 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab218648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 5µl for 105-8 cells. |
Target
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Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. -
Involvement in disease
Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
Multiple sclerosis -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
Domain
The first PTPase domain interacts with SKAP1. -
Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 457607 Chimpanzee
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
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Alternative names
- B220 antibody
- CD45 antibody
- CD45 antigen antibody
see all
Images
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Flow Cytometry - redFluor™ 710 Anti-CD45RA antibody [HI100] (ab218648)
Flow Cytometry analysis of Human peripheral blood lymphocytes labeling CD45RA with ab218648 at 5 µL (0.25 µg) per reaction (solid line) or 0.25 µg redFluor™ 710 Mouse IgG2b isotype control (dashed line).
Datasheets and documents
References (0)
ab218648 has not yet been referenced specifically in any publications.
