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Immunology Adaptive Immunity B Cells CD
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redFluor™ 710 Anti-CD45RA antibody [HI100] (ab218648)

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Flow Cytometry - redFluor™ 710 Anti-CD45RA antibody [HI100] (ab218648)

    Key features and details

    • redFluor™ 710 Mouse monoclonal [HI100] to CD45RA
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: redFluor™ 710
    • Isotype: IgG2b

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    Overview

    • Product name

      redFluor™ 710 Anti-CD45RA antibody [HI100]
      See all CD45RA primary antibodies
    • Description

      redFluor™ 710 Mouse monoclonal [HI100] to CD45RA
    • Host species

      Mouse
    • Conjugation

      redFluor™ 710
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Chimpanzee
    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • FC; Human peripheral blood lymphocytes.
    • General notes

      Conjugated to redFluor™ 710. RedFluor™ 710 dye is excited by the red (633-647 nm) laser and has a peak emission of 710 nm. The recommended band pass filter for this dye is 710/50. RedFluor™ 710 can be used as an alternative for Alexa Fluor® 700. Confirm that your cytometer is configured to detect this fluorochrome.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      ab218648 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
    • Clonality

      Monoclonal
    • Clone number

      HI100
    • Isotype

      IgG2b
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Adaptive Immunity
      • B Cells
      • CD
      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Immunology
      • Cell Type Markers
      • CD
      • Non-lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage

    Associated products

    • Alternative Versions

      • PerCP/Cy5.5® Anti-CD45RA antibody [HI100], prediluted (ab155362)
    • Isotype control

      • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab218648 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 5µl for 105-8 cells.
    Notes
    Flow Cyt
    Use 5µl for 105-8 cells.

    Target

    • Function

      Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
    • Involvement in disease

      Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
      Multiple sclerosis
    • Sequence similarities

      Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 tyrosine-protein phosphatase domains.
    • Domain

      The first PTPase domain interacts with SKAP1.
    • Post-translational
      modifications

      Heavily N- and O-glycosylated.
    • Cellular localization

      Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Target information above from: UniProt accession P08575 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 457607 Chimpanzee
      • Entrez Gene: 5788 Human
      • Omim: 151460 Human
      • SwissProt: P08575 Human
      • Unigene: 654514 Human
      • Alternative names

        • B220 antibody
        • CD45 antibody
        • CD45 antigen antibody
        • CD45R antibody
        • EC 3.1.3.48 antibody
        • GP180 antibody
        • L-CA antibody
        • LCA antibody
        • Leukocyte common antigen antibody
        • Loc antibody
        • Ly-5 antibody
        • LY5 antibody
        • Ly5, homolog of antibody
        • Lymphocyte antigen 5 antibody
        • Lyt-4 antibody
        • Protein tyrosine phosphatase, receptor type C antibody
        • Protein tyrosine phosphatase, receptor type, C antibody
        • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
        • Ptprc antibody
        • PTPRC_HUMAN antibody
        • Receptor-type tyrosine-protein phosphatase C antibody
        • RT7 antibody
        • T200 antibody
        • T200 glycoprotein antibody
        • T200 leukocyte common antigen antibody
        see all

      Images

      • Flow Cytometry - redFluor™ 710 Anti-CD45RA antibody [HI100] (ab218648)
        Flow Cytometry - redFluor™ 710 Anti-CD45RA antibody [HI100] (ab218648)

        Flow Cytometry analysis of Human peripheral blood lymphocytes labeling CD45RA with ab218648 at 5 µL (0.25 µg) per reaction (solid line) or 0.25 µg redFluor™ 710 Mouse IgG2b isotype control (dashed line).

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab218648? Please let us know so that we can cite the reference in this datasheet.

      ab218648 has not yet been referenced specifically in any publications.

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