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    reduced-glutathione-gsh-assay-kit-fluorometric-ab235670.pdf

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Signal Transduction Metabolism Amino Acids
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Reduced Glutathione (GSH) Assay Kit (Fluorometric) (ab235670)

  • Datasheet
  • SDS
  • Protocol Booklet
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  • Example Data
  • Example Data
  • Example Data
  • Example Data
  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Reduced Glutathione (GSH) Assay Kit (Fluorometric)
    See all Glutathione kits
  • Detection method

    Fluorescent
  • Sample type

    Cell culture extracts, Other biological fluids, Tissue Homogenate
  • Assay type

    Quantitative
  • Species reactivity


    Predicted to work with: Mammals
  • Product overview

    Reduced Glutathione (GSH) Assay kit (ab235670) is a simple, specific and sensitive method for GSH quantification in various biological samples/preparations.


    This kit is based on a specific enzymatic cycling method in the presence of GSH and a fluorophore. The reduction of the fluorophore produces a stable fluorescent product, the fluorescence of which is directly proportional to the amount of GSH in the sample and can be followed kinetically (Ex/Em=535/587 nm). The assay is simple, reproducible and can specifically detect as low as 8 pmol/well of reduced form of Glutathione (GSH) in a 100 μl reaction.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Enzyme Mix A 1 x 15µl
    Enzyme Mix B 1 x 120µl
    Enzyme Mix C 1 vial
    GSH Assay Buffer 1 x 50ml
    GSH Probe 1 x 0.4ml
    GSH Standard  1 vial
    Substrate Mix A 1 x 1ml
    Substrate Mix B 1 vial
    Sulfosalicylic Acid (SSA, 1 gram) 1 unit
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
  • Relevance

    Glutathione is a small peptide composed of three amino acids: cysteine, glutamic acid, and glycine and is present in tissues in concentrations as high as one millimolar. It contains an unusual peptide linkage between the amine group of cysteine and the carboxyl group of the glutamate side chain. Glutathione is involved in detoxification, it binds to toxins, such as heavy metals, solvents, and pesticides, and transforms them into a form that can be excreted in urine or bile. It is also an important antioxidant, helping to maintain the -SH groups of proteins in their reduced form. Chronic functional glutathione deficiency is associated with glucose 6-phosphate dehydrogenase deficiency, immune disorders, an increased incidence of malignancies, and in the case of HIV disease, probably accelerated pathogenesis of the disease. Acute manifestations of functional glutathione deficiency can be seen in those who have taken an overdosage of acetaminophen (paracetamol). This results in depletion of glutathione in the hepatocytes, leading to liver failure and death.
  • Alternative names

    • Glutathione
    • GSH
    • Oxidised glutathione
    • Reduced glutathione

Images

  • Example Data
    Example Data

    GSH standard curve.

  • Example Data
    Example Data

    Assay Specificity: Measurement of GSH (100 pmol) and GSSG (1000 pmol). The kit can effectively discriminate reduced GSH and oxidized GSSG forms.

  • Example Data
    Example Data

    Measurement of GSH in human plasma (Spiked and un-spiked samples).

  • Example Data
    Example Data

    Measurement of GSH in mouse Liver (1.2 μg protein), HepG2 cell extract (0.6 μg protein), Jurkat cell extract (1 μg protein) and human plasma (10 μl, 1:4 dilution).

Protocols

  • Protocol Booklet

Datasheets and documents

    • Datasheet
    • SDS
  • References

    ab235670 has not yet been referenced specifically in any publications.

    Publishing research using ab235670? Please let us know so that we can cite the reference in this datasheet.

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