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Neuroscience Cell Adhesion Proteins ECM Proteins
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Anti-Reelin antibody (ab138370)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody (ab138370)

    Key features and details

    • Rabbit polyclonal to Reelin
    • Suitable for: IHC-P
    • Reacts with: Mouse
    • Isotype: IgG

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    Overview

    • Product name

      Anti-Reelin antibody
      See all Reelin primary antibodies
    • Description

      Rabbit polyclonal to Reelin
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Mouse
      Predicted to work with: Rat, Rabbit, Horse, Chicken, Cow, Dog, Human, Pig, Macaque monkey, Gorilla
    • Immunogen

      Synthetic peptide corresponding to Mouse Reelin aa 3400 to the C-terminus conjugated to keyhole limpet haemocyanin.
      Database link: Q60841

    • Positive control

      • This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal brain.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Cell Adhesion Proteins
      • ECM Proteins
      • Neuroscience
      • Neurology process
      • Growth and Development
      • Axonal Guidance Proteins
      • Neuroscience
      • Neurology process
      • Neurogenesis

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Reelin protein (His tag) (ab236169)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab138370 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P (1)
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Notes
    IHC-P
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Function

      Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation.
    • Tissue specificity

      Abundantly produced during brain ontogenesis by the Cajal-Retzius cells and other pioneer neurons located in the telencephalic marginal zone and by granule cells of the external granular layer of the cerebellum. In adult brain, preferentially expressed in GABAergic interneurons of prefrontal cortices, temporal cortex, hippocampus and glutamatergic granule cells of cerebellum. Expression is reduced to about 50% in patients with schizophrenia. Also expressed in fetal and adult liver.
    • Involvement in disease

      Lissencephaly 2
    • Sequence similarities

      Belongs to the reelin family.
      Contains 16 BNR repeats.
      Contains 8 EGF-like domains.
      Contains 1 reelin domain.
    • Developmental stage

      Expressed in fetal and postnatal brain and liver. Expression in postnatal human brain is high in the cerebellum.
    • Domain

      The basic C-terminal region is essential for secretion.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession P78509 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5649 Human
      • Entrez Gene: 19699 Mouse
      • Entrez Gene: 24718 Rat
      • Omim: 600514 Human
      • SwissProt: P78509 Human
      • SwissProt: Q60841 Mouse
      • SwissProt: P58751 Rat
      • Unigene: 655654 Human
      • Unigene: 425236 Mouse
      • Unigene: 98353 Rat
      see all
    • Alternative names

      • LIS2 antibody
      • PRO1598 antibody
      • Reeler antibody
      • Reelin antibody
      • RELN antibody
      • RELN_HUMAN antibody
      • RL antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody (ab138370)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reelin antibody (ab138370)

      IHC image of Reelin staining in Mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab138370, 1µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab138370? Please let us know so that we can cite the reference in this datasheet.

    ab138370 has been referenced in 1 publication.

    • Yang X  et al. A novel mechanism of memory loss in Alzheimer's disease mice via the degeneration of entorhinal-CA1 synapses. Mol Psychiatry 23:199-210 (2018). PubMed: 27671476

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Reelin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (cerebellum)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6, 100*C, 30min
    Permeabilization
    No
    Specification
    cerebellum
    Blocking step
    H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 30 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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