Overview

  • Product name

    Anti-Reelin antibody [EPR3330(2)]
    See all Reelin primary antibodies
  • Description

    Rabbit monoclonal [EPR3330(2)] to Reelin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Reelin aa 3250-3350. The exact sequence is proprietary.

  • Positive control

    • HepG2, C6, and Human fetal brain lysates.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab139691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 388 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation.
    • Tissue specificity

      Abundantly produced during brain ontogenesis by the Cajal-Retzius cells and other pioneer neurons located in the telencephalic marginal zone and by granule cells of the external granular layer of the cerebellum. In adult brain, preferentially expressed in GABAergic interneurons of prefrontal cortices, temporal cortex, hippocampus and glutamatergic granule cells of cerebellum. Expression is reduced to about 50% in patients with schizophrenia. Also expressed in fetal and adult liver.
    • Involvement in disease

      Lissencephaly 2
    • Sequence similarities

      Belongs to the reelin family.
      Contains 16 BNR repeats.
      Contains 8 EGF-like domains.
      Contains 1 reelin domain.
    • Developmental stage

      Expressed in fetal and postnatal brain and liver. Expression in postnatal human brain is high in the cerebellum.
    • Domain

      The basic C-terminal region is essential for secretion.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • LIS2 antibody
      • PRO1598 antibody
      • Reeler antibody
      • Reelin antibody
      • RELN antibody
      • RELN_HUMAN antibody
      • RL antibody
      see all

    Images

    • All lanes : Anti-Reelin antibody [EPR3330(2)] (ab139691) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : C6 cell lysate
      Lane 3 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 388 kDa

    References

    This product has been referenced in:

    • Valko PO  et al. In search of cerebrospinal fluid biomarkers of fatigue in multiple sclerosis: A proteomics study. J Sleep Res N/A:e12721 (2018). Read more (PubMed: 29961995) »
    • Fathi E  et al. The Effects of Alpha Boswellic Acid on Reelin Expression and Tau Phosphorylation in Human Astrocytes. Neuromolecular Med N/A:N/A (2016). WB ; Human . Read more (PubMed: 27567921) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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