Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3330(2)] to Reelin
- Suitable for: WB
- Reacts with: Rat, Human
Product nameAnti-Reelin antibody [EPR3330(2)]
See all Reelin primary antibodies
DescriptionRabbit monoclonal [EPR3330(2)] to Reelin
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human Reelin aa 3250-3350. The exact sequence is proprietary.
- HepG2, C6, and Human fetal brain lysates.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab139691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 388 kDa.|
FunctionExtracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation.
Tissue specificityAbundantly produced during brain ontogenesis by the Cajal-Retzius cells and other pioneer neurons located in the telencephalic marginal zone and by granule cells of the external granular layer of the cerebellum. In adult brain, preferentially expressed in GABAergic interneurons of prefrontal cortices, temporal cortex, hippocampus and glutamatergic granule cells of cerebellum. Expression is reduced to about 50% in patients with schizophrenia. Also expressed in fetal and adult liver.
Involvement in diseaseLissencephaly 2
Sequence similaritiesBelongs to the reelin family.
Contains 16 BNR repeats.
Contains 8 EGF-like domains.
Contains 1 reelin domain.
Developmental stageExpressed in fetal and postnatal brain and liver. Expression in postnatal human brain is high in the cerebellum.
DomainThe basic C-terminal region is essential for secretion.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- LIS2 antibody
- PRO1598 antibody
- Reeler antibody
All lanes : Anti-Reelin antibody [EPR3330(2)] (ab139691) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 388 kDa
ab139691 has been referenced in 2 publications.
- Valko PO et al. In search of cerebrospinal fluid biomarkers of fatigue in multiple sclerosis: A proteomics study. J Sleep Res N/A:e12721 (2018). PubMed: 29961995
- Fathi E et al. The Effects of Alpha Boswellic Acid on Reelin Expression and Tau Phosphorylation in Human Astrocytes. Neuromolecular Med N/A:N/A (2016). WB ; Human . PubMed: 27567921