Overview

  • Product name

    Anti-Reelin antibody [G10]
    See all Reelin primary antibodies
  • Description

    Mouse monoclonal [G10] to Reelin
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC (PFA fixed), ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fusion protein, corresponding to amino acids 164-496 of Mouse Reelin

  • Positive control

    • Mouse liver or kidney. Rat brain lysate and Mouse brain membrane lysate. Mouse embryonic cerebral cortex.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab78540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 388 kDa.
IHC (PFA fixed) Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Target

  • Function

    Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation.
  • Tissue specificity

    Abundantly produced during brain ontogenesis by the Cajal-Retzius cells and other pioneer neurons located in the telencephalic marginal zone and by granule cells of the external granular layer of the cerebellum. In adult brain, preferentially expressed in GABAergic interneurons of prefrontal cortices, temporal cortex, hippocampus and glutamatergic granule cells of cerebellum. Expression is reduced to about 50% in patients with schizophrenia. Also expressed in fetal and adult liver.
  • Involvement in disease

    Lissencephaly 2
  • Sequence similarities

    Belongs to the reelin family.
    Contains 16 BNR repeats.
    Contains 8 EGF-like domains.
    Contains 1 reelin domain.
  • Developmental stage

    Expressed in fetal and postnatal brain and liver. Expression in postnatal human brain is high in the cerebellum.
  • Domain

    The basic C-terminal region is essential for secretion.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • LIS2 antibody
    • PRO1598 antibody
    • Reeler antibody
    • Reelin antibody
    • RELN antibody
    • RELN_HUMAN antibody
    • RL antibody
    see all

Images

  • ab78540, at 1/1000 dilution staining Reelin in embryonic cerebral cortex at 15 days in utero, with the pial surface above and the ventricle below. The positive zone is the Cajal-Retzius cell layer in the marginal zone.

    This image was generated using the ascites version of the product.

  • All lanes : Anti-Reelin antibody [G10] (ab78540) at 1/500 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Mouse brain membrane lysate

    Secondary
    All lanes : Goat anti-mouse secondary antibody
    conjugated to HRP

    Predicted band size: 388 kDa
    Observed band size: 388 kDa
    Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands.



    This image was generated using the ascites version of the product.

  • Immunolocalization of Reelin (red) in the cortex of e14.5 mouse CNS using ab78540 at 1/1000 dilution. The blue stain is Hoechst nuclear counter stain.

    This image was generated using the ascites version of the product.

  • Anti-Reelin antibody [G10] (ab78540) at 1/500 dilution + Rat brain lysate at 10 µg

    Secondary
    Goat anti-mouse secondary
    antibody conjugated to HRP

    Predicted band size: 388 kDa
    Observed band size: 388 kDa



    This image was generated using the ascites version of the product.

References

This product has been referenced in:

See all 12 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain section - 50um)
Antigen retrieval step
None
Permeabilization
Yes - PBS-T 3 x 5mn
Specification
Brain section - 50um
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 13 2019

Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: RT°C
Sample
Mouse Cultured Cells (Primary cortical cells)
Specification
Primary cortical cells
Permeabilization
Yes - Acetone - 10 minutes
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 24 2013

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