Key features and details
- Mouse monoclonal [S345-51] to REEP1
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG2b
Product nameAnti-REEP1 antibody [S345-51]
See all REEP1 primary antibodies
DescriptionMouse monoclonal [S345-51] to REEP1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- ICC/IF: SK-N-BE cells. WB: Rat brain lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab242004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 22 kDa.|
FunctionMay enhance the cell surface expression of odorant receptors.
Involvement in diseaseDefects in REEP1 are the cause of spastic paraplegia autosomal dominant type 31 (SPG31) [MIM:610250]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
Sequence similaritiesBelongs to the DP1 family.
Cellular localizationMitochondrion membrane.
- Information by UniProt
- C2orf23 antibody
- Chromosome 2 open reading frame 23 antibody
- FLJ13110 antibody
Immunocytochemistry/Immunofluorescence analysis of SK-N-BE (Human neuroblastoma cell line) cells labeling REEP1 using ab242004 at 1:100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at RT.
Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min RT, 5 min RT.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab242004 (D) Composite.
Anti-REEP1 antibody [S345-51] (ab242004) at 1/1000 dilution (16 hours at +4degC) + Rat brain lysate at 20 µg
Goat Anti-Mouse IgG: HRP, 1 hour at RT
Developed using the ECL technique.
Predicted band size: 22 kDa
Exposure time: 6 minutes
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab242004 has not yet been referenced specifically in any publications.