Key features and details
- Mouse monoclonal [S326D-2] to REEP2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2a
Product nameAnti-REEP2 antibody [S326D-2]
See all REEP2 primary antibodies
DescriptionMouse monoclonal [S326D-2] to REEP2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
- WB: COS over expressing REEP2-His. ICC/IF: SK-N-BE cells.
This product was previously labelled as C5orf19
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab242000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 28 kDa.|
FunctionMay enhance the cell surface expression of odorant receptors.
Tissue specificityDetected in brain, heart and skeletal muscle, and at low levels in placenta, kidney and pancreas.
Sequence similaritiesBelongs to the DP1 family.
- Information by UniProt
- C5orf19 antibody
- Chromosome 5 open reading frame 19 antibody
- Receptor accessory protein 2 antibody
4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for REEP2 (green, C) using ab242000 at 1/100 dilution for 1 hour at room temperature in ICC/IF. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1/100 dilution at a 1 hr at RT. Actin is detected with Phalloidin Texas Red F-Actin stain (red, B). The nuclear counterstain is DAPI (blue, A). (D) is a composite image.
All lanes : Anti-REEP2 antibody [S326D-2] (ab242000) at 1/1000 dilution
Lane 1 : COS overexpressing REEP1-His cell lysate at 15 µg
Lane 2 : COS overexpressing REEP2-His cell lysate
Lane 3 : COS overexpressing REEP6-His cell lysate
All lanes : Goat Anti-Mouse IgG: HRP at 1/200 dilution
Developed using the ECL technique.
Predicted band size: 28 kDa
Exposure time: 6 minutes
Primary incubation for 16 hours at 4°C.
Secondary incubation for 1 hr at RT.
Blocked for 1 hr at RT.
Note: C5orf19 = REEP2
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab242000 has not yet been referenced specifically in any publications.