Key features and details
- Rabbit polyclonal to REEP5
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-REEP5 antibody
See all REEP5 primary antibodies
DescriptionRabbit polyclonal to REEP5
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Orangutan
- WB: Mouse kidney and lung tissue lysate; Jurkat, MCF7, K562 and HeLa whole cell lysate (ab150035). IHC-P: Human colon cancer and skin tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- HeLa whole cell lysate (ab150035)
- Mouse lung normal tissue lysate - total protein (ab29297)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- MCF7 whole cell lysate (ab3871)
- Jurkat whole cell lysate (ab7899)
- K562 whole cell lysate (ab7911)
Our Abpromise guarantee covers the use of ab230115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 21 kDa.|
|IHC-P||1/20 - 1/200.|
RelevanceMay promote functional cell surface expression of olfactory receptors.
Cellular localizationCell Membrane
- C5orf18 antibody
- D5S346 antibody
- Deleted in polyposis 1 antibody
All lanes : Anti-REEP5 antibody (ab230115) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 5 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Paraffin-embedded human colon cancer tissue stained for REEP5 using ab230115 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human skin tissue stained for REEP5 using ab230115 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230115 has not yet been referenced specifically in any publications.