Recombinant
RabMAb

Anti-REEP5 antibody [EPR11114(B)] (ab167405)

Abreviews (3) Submit a Question

Overview

  • Product name
    Anti-REEP5 antibody [EPR11114(B)]
    See all REEP5 primary antibodies
  • Description
    Rabbit monoclonal [EPR11114(B)] to REEP5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to amino acids in Human REEP5 (UniProt ID: Q00765).

  • Positive control
    • MCF7, K562, Jurkat and HeLa cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    Images

    • Western blot - Anti-REEP5 antibody [EPR11114(B)] (ab167405)
      Western blot - Anti-REEP5 antibody [EPR11114(B)] (ab167405)
      All lanes : Anti-REEP5 antibody [EPR11114(B)] (ab167405) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : K652 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 21 kDa

    References

    ab167405 has not yet been referenced specifically in any publications.

    Publishing research using ab167405? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    3 reviews or Q&A

    10
    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence abreview for Anti-REEP5 antibody [EPR11114(B)]

     Excellent
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Monkey Cell (Kiney)
    Permeabilization
    Yes - 0.1% troton x-100
    Specification
    Kiney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
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    Abcam user community

    Verified customer

    Submitted Jun 14 2016

    Western blot abreview for Anti-REEP5 antibody [EPR11114(B)]

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Monkey Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (12)
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Abcam user community

    Verified customer

    Submitted Feb 16 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-REEP5 antibody [EPR11114(B)]

     Average
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.3 % Triton X 100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More
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    Abcam user community

    Verified customer

    Submitted Feb 11 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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