Recombinant
RabMAb

Anti-REEP5 antibody [EPR11114(B)] (ab167405)

Overview

  • Product name
    Anti-REEP5 antibody [EPR11114(B)]
    See all REEP5 primary antibodies
  • Description
    Rabbit monoclonal [EPR11114(B)] to REEP5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to amino acids in Human REEP5 (UniProt ID: Q00765).

  • Positive control
    • MCF7, K562, Jurkat and HeLa cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-REEP5 antibody [EPR11114(B)] (ab167405) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : K652 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 21 kDa

    References

    ab167405 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Monkey Cell (Kiney)
    Permeabilization
    Yes - 0.1% troton x-100
    Specification
    Kiney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jun 14 2016

    Application
    Western blot
    Sample
    Monkey Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (12)
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 16 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.3 % Triton X 100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 11 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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