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RecombinantRabMAb

Recombinant Anti-REEP5 antibody [EPR11114(B)] (ab167405)

  • Datasheet
  • SDS
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Western blot - Anti-REEP5 antibody [EPR11114(B)] (ab167405)
  • Anti-REEP5 antibody [EPR11114(B)] (ab167405)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11114(B)] to REEP5
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-REEP5 antibody [EPR11114(B)]
    See all REEP5 primary antibodies
  • Description

    Rabbit monoclonal [EPR11114(B)] to REEP5
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human REEP5.

  • Positive control

    • MCF7, K562, Jurkat and HeLa whole cell lysate (ab150035)
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11114(B)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • More GPCR
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Sensory System
    • Olfactory system

Associated products

  • Alternative Versions

    • Anti-REEP5 antibody [EPR11114(B)] - BSA and Azide free (ab249420)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab167405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Relevance

      May promote functional cell surface expression of olfactory receptors.
    • Cellular localization

      Cell Membrane
    • Database links

      • Entrez Gene: 7905 Human
      • Entrez Gene: 13476 Mouse
      • Entrez Gene: 364838 Rat
      • Omim: 125265 Human
      • SwissProt: Q00765 Human
      • SwissProt: Q60870 Mouse
      • SwissProt: B2RZ37 Rat
      • Unigene: 429608 Human
      • Unigene: 280075 Mouse
      • Unigene: 40477 Rat
      see all
    • Alternative names

      • C5orf18 antibody
      • D5S346 antibody
      • Deleted in polyposis 1 antibody
      • DP1 antibody
      • Polyposis coli region hypothetical protein DP1 antibody
      • Polyposis locus protein 1 antibody
      • Protein TB2 antibody
      • Receptor accessory protein 5 antibody
      • Receptor expression enhancing protein 5 antibody
      • TB2 antibody
      • TB2 protein antibody
      • YOP1 antibody
      see all

    Images

    • Western blot - Anti-REEP5 antibody [EPR11114(B)] (ab167405)
      Western blot - Anti-REEP5 antibody [EPR11114(B)] (ab167405)
      All lanes : Anti-REEP5 antibody [EPR11114(B)] (ab167405) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : K652 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 21 kDa

    • Anti-REEP5 antibody [EPR11114(B)] (ab167405)
      Anti-REEP5 antibody [EPR11114(B)] (ab167405)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab167405? Please let us know so that we can cite the reference in this datasheet.

    ab167405 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-REEP5 antibody [EPR11114(B)]

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Monkey Cell (Kiney)
    Permeabilization
    Yes - 0.1% troton x-100
    Specification
    Kiney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 14 2016

    Western blot abreview for Anti-REEP5 antibody [EPR11114(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Monkey Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (12)
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 16 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-REEP5 antibody [EPR11114(B)]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.3 % Triton X 100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 11 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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