Overview

  • Product name

    Anti-Ref2P antibody
  • Description

    Rabbit polyclonal to Ref2P
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide corresponding to Drosophila melanogaster Ref2P aa 200-300 conjugated to keyhole limpet haemocyanin.
    Database link: P14199

  • Positive control

    • This antibody gave a positive signal in Atg8a mutant Fruit Flies (D.melanogaster).

Properties

Applications

Our Abpromise guarantee covers the use of ab178440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 92 kDa (predicted molecular weight: 65 kDa).

Target

  • Function

    Implicated in sigma rhabdovirus multiplication and necessary for male fertility.
  • Sequence similarities

    Contains 1 OPR domain.
    Contains 1 UBA domain.
    Contains 1 ZZ-type zinc finger.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

    • Alternative names

      • Protein ref(2)P antibody
      • ref(2)P antibody
      • REF2P_DROME antibody
      • Refractory to sigma P antibody

    Images

    • All lanes : Anti-Ref2P antibody (ab178440) at 1/500 dilution (Blocking 60 minutes in TBS+0.1% Tween-20 + 5% non-fat milk)

      Lane 1 : Wt adult drosophila lysate
      Lane 2 : Atg8a mutant adult drosophila lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-rabbit HRP at 1/10000 dilution

      Predicted band size: 65 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?


      Exposure time: 2 seconds


      Gel 8% acrylamide, transferred onto PVDF membrane for 60 minutes at 100V in 10% ethanol buffer. Anti-Ref2P antibody 1:500 incubated overnight at 4ºC, with gentle agitation in TBS + 0.1% Tween-20.

    • All lanes : Anti-Ref2P antibody (ab178440) at 1/100 dilution (Milk blocked)

      Lanes 1-3 : Atg8a mutant fruit flies (D.melanogaster)
      Lanes 4-6 : Fruit flies (D.melanogaster) expressing ubiquitously Ref(2)p RNAi

      Predicted band size: 65 kDa
      Observed band size: 92 kDa why is the actual band size different from the predicted?



      The bands observed at the bottom of the gel represent Tubulin and were used as a loading control within this experiment.

    References

    This product has been referenced in:

    • Guo T  et al. The autophagy-related gene Atg101 in Drosophila regulates both neuron and midgut homeostasis. J Biol Chem 294:5666-5676 (2019). Read more (PubMed: 30760524) »
    • Nilangekar K  et al. Generation and Characterization of Germline-Specific Autophagy and Mitochondrial Reactive Oxygen Species Reporters in Drosophila. Front Cell Dev Biol 7:47 (2019). Read more (PubMed: 31001531) »
    See all 10 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    IHC - Wholemount
    Sample
    Drosophila melanogaster Tissue (Thoracic muscles)
    Specification
    Thoracic muscles

    Megumi Mori

    Verified customer

    Submitted May 22 2019

    Application
    IHC - Wholemount
    Sample
    Drosophila melanogaster Tissue (adult brain)
    Specification
    adult brain

    Dr. Anne-Claire Jacomin

    Verified customer

    Submitted Feb 21 2018

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