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IHC - Wholemount
Drosophila melanogaster Tissue (adult brain)
Other product details
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.1% Triton X-100 + 0.3% BSA
Non-Abcam antibody was used: anti-rabbit Alexa 568
Host species: Goat
Conjugation: Alexa Fluor® 568
Fixation: 4% PFA in PBS, 30min at room temperature.
Washes performed in PBS + 0.1% Tween-20.
Secondary antibody incubation: 2hrs at room temperature.
Nuclei stained with Hoechst 33342.
Dr. Anne-Claire Jacomin
Submitted Feb 21 2018