IHC - Wholemount abreview for Anti-Ref2P antibody

Excellent
Abreviews
Application
IHC - Wholemount
Sample
Drosophila melanogaster Tissue (adult brain)
Specification
adult brain

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.1% Triton X-100 + 0.3% BSA
Dilution
1/400

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: anti-rabbit Alexa 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568

Additional data

Additional Notes
Fixation: 4% PFA in PBS, 30min at room temperature. Washes performed in PBS + 0.1% Tween-20. Secondary antibody incubation: 2hrs at room temperature. Nuclei stained with Hoechst 33342.

Dr. Anne-Claire Jacomin

Verified customer

Submitted Feb 21 2018

Sign up