Anti-REG1 alpha antibody (ab231092)
Key features and details
- Rabbit polyclonal to REG1 alpha
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-REG1 alpha antibody
See all REG1 alpha primary antibodies -
Description
Rabbit polyclonal to REG1 alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Rat REG1 alpha aa 1 to the C-terminus. Full length mature chain, expressed in E.coli. N-terminal tags.
Database link: P10758 -
Positive control
- IHC-P: Rat intestine tissue. WB: Rat and mouse pancreas lysates; Recombinant rat REG1 alpha protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231092 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 19 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 19 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration. -
Tissue specificity
In pancreatic acinar cells and, in lower levels, in brain. Enhanced expression of PSP-related transcripts and intraneuronal accumulation of PSP-like proteins is found in brain from Alzheimer disease and Down syndrome patients. -
Sequence similarities
Contains 1 C-type lectin domain. -
Developmental stage
High expression levels in fetal and infant brains; much lower in adult brains. -
Post-translational
modificationsThe composition of the O-linked carbohydrate on Thr-27 is complex and varied. In the crystallographic structure, the attached sugar appears to be N-acetylglucosamine, typical of an intracellular protein, rather than N-acetylgalactosamine. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 24714 Rat
- SwissProt: P43137 Mouse
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Alternative names
- ICRF antibody
- Islet cells regeneration factor antibody
- Islet of Langerhans regenerating protein antibody
see all
Images
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Formalin-fixed, paraffin-embedded rat intestine tissue stained for REG1 alpha using ab231092 at 20 µg/ml in immunohistochemical analysis, followed by DAB staining.
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All lanes : Anti-REG1 alpha antibody (ab231092) at 2 µg/ml
Lane 1 : Rat pancreas lysate
Lane 2 : Mouse pancreas lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit antibody at 1/1000 dilution
Predicted band size: 19 kDa -
Anti-REG1 alpha antibody (ab231092) at 2 µg/ml + Recombinant rat REG1 alpha protein
Predicted band size: 19 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231092 has not yet been referenced specifically in any publications.