Key features and details
- Rabbit polyclonal to REG1 alpha
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-REG1 alpha antibody
See all REG1 alpha primary antibodies
DescriptionRabbit polyclonal to REG1 alpha
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Recombinant full length protein (His-T7-tag) corresponding to Rat REG1 alpha aa 22-165. Full length mature chain, expressed in E.coli. N-terminal tags.
QEAEEDLPSARITCPEGSNAYSSYCYYFMEDHLSWAEADLFCQNMNSGYL VSVLSQAEGNFLASLIKESGTTAANVWIGLHDPKNNRRWHWSSGSLFLYK SWDTGYPNNSNRGYCVSVTSNSGYKKWRDNSCDAQLSFVCKFKA
Database link: P10758
- IHC-P: Rat intestine tissue. WB: Rat and mouse pancreas lysates; Recombinant rat REG1 alpha protein.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231092 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 19 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionMight act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration.
Tissue specificityIn pancreatic acinar cells and, in lower levels, in brain. Enhanced expression of PSP-related transcripts and intraneuronal accumulation of PSP-like proteins is found in brain from Alzheimer disease and Down syndrome patients.
Sequence similaritiesContains 1 C-type lectin domain.
Developmental stageHigh expression levels in fetal and infant brains; much lower in adult brains.
modificationsThe composition of the O-linked carbohydrate on Thr-27 is complex and varied. In the crystallographic structure, the attached sugar appears to be N-acetylglucosamine, typical of an intracellular protein, rather than N-acetylgalactosamine.
- Information by UniProt
- ICRF antibody
- Islet cells regeneration factor antibody
- Islet of Langerhans regenerating protein antibody
Formalin-fixed, paraffin-embedded rat intestine tissue stained for REG1 alpha using ab231092 at 20 µg/ml in immunohistochemical analysis, followed by DAB staining.
All lanes : Anti-REG1 alpha antibody (ab231092) at 2 µg/ml
Lane 1 : Rat pancreas lysate
Lane 2 : Mouse pancreas lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit antibody at 1/1000 dilution
Predicted band size: 19 kDa
Anti-REG1 alpha antibody (ab231092) at 2 µg/ml + Recombinant rat REG1 alpha protein
Predicted band size: 19 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231092 has not yet been referenced specifically in any publications.