Anti-REG1 alpha antibody (ab47099)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-REG1 alpha antibody
See all REG1 alpha primary antibodies -
Description
Rabbit polyclonal to REG1 alpha -
Host species
Rabbit -
Specificity
This antibody is specific for REG 1 alpha. It was tested in a sandwich ELISA with antigens: Reg 1beta, PAP=(REG III), and Reg IV. We did not detect any crossreactivity. -
Tested applications
Suitable for: IHC-P, ELISA, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant human REG 1 alpha. A 17.8 kDa protein containing 144 amino acid residues of the human REG 1 alpha and 12 additional amino acid residues, HisTag. Source of immunogen E.coli
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Positive control
- recombinant protein
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituents: 0.134% PBS, 0.58% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Immunoaffinity chromatography on a column with immobilized recombinant human REG 1 alpha. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab47099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 19 kDa). |
Target
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Function
Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration. -
Tissue specificity
In pancreatic acinar cells and, in lower levels, in brain. Enhanced expression of PSP-related transcripts and intraneuronal accumulation of PSP-like proteins is found in brain from Alzheimer disease and Down syndrome patients. -
Sequence similarities
Contains 1 C-type lectin domain. -
Developmental stage
High expression levels in fetal and infant brains; much lower in adult brains. -
Post-translational
modificationsThe composition of the O-linked carbohydrate on Thr-27 is complex and varied. In the crystallographic structure, the attached sugar appears to be N-acetylglucosamine, typical of an intracellular protein, rather than N-acetylgalactosamine. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5967 Human
- Omim: 167770 Human
- SwissProt: P05451 Human
- Unigene: 4158 Human
- Unigene: 49407 Human
- Unigene: 708865 Human
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Alternative names
- ICRF antibody
- Islet cells regeneration factor antibody
- Islet of Langerhans regenerating protein antibody
see all
Images
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Western blot - Anti-REG1 alpha antibody (ab47099)Anti-REG1 alpha antibody (ab47099) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Exposure time: 30 seconds
The band observed at 14 kDa could potentially be a cleaved form of REG1 alpha due to the presence of a 25 amino acid signal peptide.
Datasheets and documents
References
This product has been referenced in:
- Saitoh S et al. Improved Serial Sectioning Techniques for Correlative Light-Electron Microscopy Mapping of Human Langerhans Islets. Acta Histochem Cytochem 51:9-20 (2018). Read more (PubMed: 29622846) »
- Radon TP et al. Identification of a Three-Biomarker Panel in Urine for Early Detection of Pancreatic Adenocarcinoma. Clin Cancer Res 21:3512-21 (2015). Read more (PubMed: 26240291) »
