Overview

  • Product name
    Anti-REG1 alpha antibody
    See all REG1 alpha primary antibodies
  • Description
    Rabbit polyclonal to REG1 alpha
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for REG 1 alpha. It was tested in a sandwich ELISA with antigens: Reg 1beta, PAP=(REG III), and Reg IV. We did not detect any crossreactivity.
  • Tested applications
    Suitable for: IHC-P, ELISA, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human REG 1 alpha. A 17.8 kDa protein containing 144 amino acid residues of the human REG 1 alpha and 12 additional amino acid residues, HisTag. Source of immunogen E.coli

  • Positive control
    • recombinant protein

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Constituents: 0.134% PBS, 0.58% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Immunoaffinity chromatography on a column with immobilized recombinant human REG 1 alpha.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 19 kDa).

Target

  • Function
    Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration.
  • Tissue specificity
    In pancreatic acinar cells and, in lower levels, in brain. Enhanced expression of PSP-related transcripts and intraneuronal accumulation of PSP-like proteins is found in brain from Alzheimer disease and Down syndrome patients.
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Developmental stage
    High expression levels in fetal and infant brains; much lower in adult brains.
  • Post-translational
    modifications
    The composition of the O-linked carbohydrate on Thr-27 is complex and varied. In the crystallographic structure, the attached sugar appears to be N-acetylglucosamine, typical of an intracellular protein, rather than N-acetylgalactosamine.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • ICRF antibody
      • Islet cells regeneration factor antibody
      • Islet of Langerhans regenerating protein antibody
      • Lithostathine 1 alpha antibody
      • Lithostathine 1 alpha precursor antibody
      • Lithostathine antibody
      • Lithostathine-1-alpha antibody
      • MGC12447 antibody
      • P19 antibody
      • Pancreatic stone protein antibody
      • Pancreatic stone protein secretory antibody
      • Pancreatic thread protein antibody
      • Protein X antibody
      • PSP antibody
      • PSPS antibody
      • PSPS1 antibody
      • PTP antibody
      • RATRGPI antibody
      • REG antibody
      • REG-1-alpha antibody
      • Reg1 antibody
      • REG1A antibody
      • REG1A_HUMAN antibody
      • Regenerating islet derived 1 alpha antibody
      • Regenerating islet derived 1 alpha pancreatic stone protein pancreatic thread protein antibody
      • Regenerating islet-derived protein 1-alpha antibody
      • Regenerating protein I alpha antibody
      see all

    Images

    • Western blot - Anti-REG1 alpha antibody (ab47099)
      Western blot - Anti-REG1 alpha antibody (ab47099)
      Anti-REG1 alpha antibody (ab47099) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 19 kDa


      Exposure time: 30 seconds


      The band observed at 14 kDa could potentially be a cleaved form of REG1 alpha due to the presence of a 25 amino acid signal peptide.

    References

    This product has been referenced in:
    • Saitoh S  et al. Improved Serial Sectioning Techniques for Correlative Light-Electron Microscopy Mapping of Human Langerhans Islets. Acta Histochem Cytochem 51:9-20 (2018). Read more (PubMed: 29622846) »
    • Radon TP  et al. Identification of a Three-Biomarker Panel in Urine for Early Detection of Pancreatic Adenocarcinoma. Clin Cancer Res 21:3512-21 (2015). Read more (PubMed: 26240291) »

    See all 2 Publications for this product

    Publishing research using ab47099? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab47099.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up