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Anti-REG1 alpha antibody (ab47099)

  • Datasheet
  • SDS
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Western blot - Anti-REG1 alpha antibody (ab47099)

    Key features and details

    • Rabbit polyclonal to REG1 alpha
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-REG1 alpha antibody
      See all REG1 alpha primary antibodies
    • Description

      Rabbit polyclonal to REG1 alpha
    • Host species

      Rabbit
    • Specificity

      This antibody is specific for REG 1 alpha. It was tested in a sandwich ELISA with antigens: Reg 1beta, PAP=(REG III), and Reg IV. We did not detect any crossreactivity.
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Recombinant human REG 1 alpha. A 17.8 kDa protein containing 144 amino acid residues of the human REG 1 alpha and 12 additional amino acid residues, HisTag. Source of immunogen E.coli

    • Positive control

      • recombinant protein

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Constituents: 0.134% PBS, 0.58% Sodium chloride
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Immunoaffinity chromatography on a column with immobilized recombinant human REG 1 alpha.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Other Antibodies
      • Other Antibodies
      • Cancer
      • Tumor biomarkers
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab47099 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 19 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 19 kDa).

    Target

    • Function

      Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration.
    • Tissue specificity

      In pancreatic acinar cells and, in lower levels, in brain. Enhanced expression of PSP-related transcripts and intraneuronal accumulation of PSP-like proteins is found in brain from Alzheimer disease and Down syndrome patients.
    • Sequence similarities

      Contains 1 C-type lectin domain.
    • Developmental stage

      High expression levels in fetal and infant brains; much lower in adult brains.
    • Post-translational
      modifications

      The composition of the O-linked carbohydrate on Thr-27 is complex and varied. In the crystallographic structure, the attached sugar appears to be N-acetylglucosamine, typical of an intracellular protein, rather than N-acetylgalactosamine.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P05451 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5967 Human
      • Omim: 167770 Human
      • SwissProt: P05451 Human
      • Unigene: 4158 Human
      • Unigene: 49407 Human
      • Unigene: 708865 Human
      • Alternative names

        • ICRF antibody
        • Islet cells regeneration factor antibody
        • Islet of Langerhans regenerating protein antibody
        • Lithostathine 1 alpha antibody
        • Lithostathine 1 alpha precursor antibody
        • Lithostathine antibody
        • Lithostathine-1-alpha antibody
        • MGC12447 antibody
        • P19 antibody
        • Pancreatic stone protein antibody
        • Pancreatic stone protein secretory antibody
        • Pancreatic thread protein antibody
        • Protein X antibody
        • PSP antibody
        • PSPS antibody
        • PSPS1 antibody
        • PTP antibody
        • RATRGPI antibody
        • REG antibody
        • REG-1-alpha antibody
        • Reg1 antibody
        • REG1A antibody
        • REG1A_HUMAN antibody
        • Regenerating islet derived 1 alpha antibody
        • Regenerating islet derived 1 alpha pancreatic stone protein pancreatic thread protein antibody
        • Regenerating islet-derived protein 1-alpha antibody
        • Regenerating protein I alpha antibody
        see all

      Images

      • Western blot - Anti-REG1 alpha antibody (ab47099)
        Western blot - Anti-REG1 alpha antibody (ab47099)
        Anti-REG1 alpha antibody (ab47099) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 19 kDa


        Exposure time: 30 seconds


        The band observed at 14 kDa could potentially be a cleaved form of REG1 alpha due to the presence of a 25 amino acid signal peptide.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (3)

      Publishing research using ab47099? Please let us know so that we can cite the reference in this datasheet.

      ab47099 has been referenced in 3 publications.

      • Saitoh S  et al. Improved Serial Sectioning Techniques for Correlative Light-Electron Microscopy Mapping of Human Langerhans Islets. Acta Histochem Cytochem 51:9-20 (2018). PubMed: 29622846
      • Ferretti E  et al. Impaired antimicrobial response and mucosal protection induced by ibuprofen in the immature human intestine. Pediatr Res 84:813-820 (2018). PubMed: 30341414
      • Radon TP  et al. Identification of a Three-Biomarker Panel in Urine for Early Detection of Pancreatic Adenocarcinoma. Clin Cancer Res 21:3512-21 (2015). PubMed: 26240291

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