Anti-REG1 alpha antibody (ab47099)
Key features and details
- Rabbit polyclonal to REG1 alpha
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-REG1 alpha antibody
See all REG1 alpha primary antibodies -
Description
Rabbit polyclonal to REG1 alpha -
Host species
Rabbit -
Specificity
This antibody is specific for REG 1 alpha. It was tested in a sandwich ELISA with antigens: Reg 1beta, PAP=(REG III), and Reg IV. We did not detect any crossreactivity. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant human REG 1 alpha. A 17.8 kDa protein containing 144 amino acid residues of the human REG 1 alpha and 12 additional amino acid residues, HisTag. Source of immunogen E.coli
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Positive control
- recombinant protein
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.134% PBS, 0.58% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Immunoaffinity chromatography on a column with immobilized recombinant human REG 1 alpha. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 19 kDa).
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| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 19 kDa). |
Target
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Function
Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration. -
Tissue specificity
In pancreatic acinar cells and, in lower levels, in brain. Enhanced expression of PSP-related transcripts and intraneuronal accumulation of PSP-like proteins is found in brain from Alzheimer disease and Down syndrome patients. -
Sequence similarities
Contains 1 C-type lectin domain. -
Developmental stage
High expression levels in fetal and infant brains; much lower in adult brains. -
Post-translational
modificationsThe composition of the O-linked carbohydrate on Thr-27 is complex and varied. In the crystallographic structure, the attached sugar appears to be N-acetylglucosamine, typical of an intracellular protein, rather than N-acetylgalactosamine. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5967 Human
- Omim: 167770 Human
- SwissProt: P05451 Human
- Unigene: 4158 Human
- Unigene: 49407 Human
- Unigene: 708865 Human
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Alternative names
- ICRF antibody
- Islet cells regeneration factor antibody
- Islet of Langerhans regenerating protein antibody
see all
Images
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Western blot - Anti-REG1 alpha antibody (ab47099)Anti-REG1 alpha antibody (ab47099) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Exposure time: 30 seconds
The band observed at 14 kDa could potentially be a cleaved form of REG1 alpha due to the presence of a 25 amino acid signal peptide.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab47099 has been referenced in 3 publications.
- Saitoh S et al. Improved Serial Sectioning Techniques for Correlative Light-Electron Microscopy Mapping of Human Langerhans Islets. Acta Histochem Cytochem 51:9-20 (2018). PubMed: 29622846
- Ferretti E et al. Impaired antimicrobial response and mucosal protection induced by ibuprofen in the immature human intestine. Pediatr Res 84:813-820 (2018). PubMed: 30341414
- Radon TP et al. Identification of a Three-Biomarker Panel in Urine for Early Detection of Pancreatic Adenocarcinoma. Clin Cancer Res 21:3512-21 (2015). PubMed: 26240291