Overview

  • Product name

  • Description

    Rabbit polyclonal to Reg3a
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH selected from the N terminal region of Human Reg3a (NP_002571.1)

  • Positive control

    • 293 cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab95316 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 19 kDa.

Target

  • Function

    Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.
  • Tissue specificity

    Highly expressed in epidermal keratinocytes of psoriasis patients (at protein level). Constitutively expressed in intestine. Low expression is found in healthy pancreas. Overexpressed during the acute phase of pancreatitis and in some patients with chronic pancreatitis.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Domain

    The EPN motif is essential for recognition of the peptidoglycan carbohydrate backbone and for efficient bacterial killing with Glu-114 playing a key role in peptidoglycan binding and bactericidal activity.
  • Post-translational
    modifications

    Proteolytic processing by trypsin removes an inhibitory N-terminal propeptide and is essential for peptidoglycan binding and antibacterial activity.
  • Cellular localization

    Secreted. Found in the apical region of pancreatic acinar cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ18565 antibody
    • Hepatocarcinoma intestine pancreas antibody
    • Hepatointestinal pancreatic protein antibody
    • HIP antibody
    • HIP/PAP antibody
    • Human proislet peptide antibody
    • INGAP antibody
    • Pancreatic beta cell growth factor antibody
    • Pancreatitis associated protein 1 antibody
    • Pancreatitis associated protein antibody
    • Pancreatitis-associated protein 1 antibody
    • PAP antibody
    • PAP H antibody
    • PAP homologous protein antibody
    • PAP1 antibody
    • PBCGF antibody
    • Proliferation inducing protein 34 antibody
    • Proliferation inducing protein 42 antibody
    • Reg III alpha antibody
    • REG III antibody
    • Reg III-alpha antibody
    • REG-3-alpha antibody
    • REG3 antibody
    • Reg3a antibody
    • REG3A_HUMAN antibody
    • Regenerating islet derived 3 alpha antibody
    • Regenerating islet derived protein 3 alpha precursor antibody
    • Regenerating islet-derived protein 3-alpha 15 kDa form antibody
    • Regenerating islet-derived protein III-alpha antibody
    see all

Images

  • Anti-Reg3a antibody (ab95316) at 1/100 dilution (in TTBS/1% NFDM for 1 hr. at room temperature) + 293 cell line lysate at 35 µg

    Secondary
    Enzyme-linked secondary antibody (typically horseradish peroxidase or alkaline phosphatase) in TTBS/1% NFDM for 30 min. at room temperature.

    Predicted band size: 19 kDa

References

This product has been referenced in:

  • Murtha MJ  et al. Insulin receptor signaling regulates renal collecting duct and intercalated cell antibacterial defenses. J Clin Invest 128:5634-5646 (2018). Read more (PubMed: 30418175) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

The immunogen that generated the REG3A antibody ab95316 shares 69.2% sequence homology with UniProt Q6UW15, human REG3G, http://www.uniprot.org/uniprot/Q6UW15. So, there is a possibility of cross-reaction with REG3G. We unfortunately do not have any data confirming specificity for REG3A.

Read More

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