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    reg3g-antibody-ab198216.pdf

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Immunology Secreted Molecules Other secreted molecules
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Anti-REG3G antibody (ab198216)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (6)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (ab198216)
  • Western blot - Anti-REG3G antibody (ab198216)

Key features and details

  • Rabbit polyclonal to REG3G
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-REG3G antibody
    See all REG3G primary antibodies
  • Description

    Rabbit polyclonal to REG3G
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Fusion protein corresponding to Human REG3G (internal sequence). Fusion protein corresponding to a region derived from internal residues of human REG3G. The identity of the protein fusion partner is GST.
    Database link: Q6UW15

  • Positive control

    • WB: Human brain malignant glioma, mouse kidney and human kidney tissue lysates. IHC-P: Human colon cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Secreted Molecules
    • Other secreted molecules

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse kidney normal tissue lysate - total protein (ab29305)
  • Recombinant Protein

    • Recombinant Human REG3G protein (ab192295)
  • Related Products

    • Recombinant Human REG3G protein (ab192295)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab198216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Rat
Application Abreviews Notes
IHC-P
1/40 - 1/200.
WB
1/250 - 1/2000. Predicted molecular weight: 19 kDa.
Notes
IHC-P
1/40 - 1/200.
WB
1/250 - 1/2000. Predicted molecular weight: 19 kDa.

Target

  • Function

    Might be a stress protein involved in the control of bacterial proliferation.
  • Tissue specificity

    Expressed almost exclusively in the pancreas. Also expressed in testis. Not found in small intestine.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q6UW15 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 130120 Human
    • Entrez Gene: 19695 Mouse
    • Entrez Gene: 24620 Rat
    • Omim: 609933 Human
    • SwissProt: Q6UW15 Human
    • SwissProt: O09049 Mouse
    • SwissProt: P42854 Rat
    • Unigene: 447084 Human
    • Alternative names

      • LPPM429 antibody
      • Pancreatitis-associated protein 1B antibody
      • Pancreatitis-associated protein IB antibody
      • PAP IB antibody
      • PAP-1B antibody
      • PAP1B antibody
      • PAPIB antibody
      • REG III antibody
      • Reg III-gamma antibody
      • REG-3-gamma antibody
      • REG3G antibody
      • REG3G_HUMAN antibody
      • Regenerating islet derived 3 gamma antibody
      • Regenerating islet-derived protein 3-gamma antibody
      • Regenerating islet-derived protein III-gamma antibody
      • UNQ429 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (ab198216)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3G antibody (ab198216)

      Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling REG3G with ab198216 at 1/40 dilution.

    • Western blot - Anti-REG3G antibody (ab198216)
      Western blot - Anti-REG3G antibody (ab198216)
      All lanes : Anti-REG3G antibody (ab198216) at 1/250 dilution

      Lane 1 : Human brain malignant glioma lysate
      Lane 2 : Mouse kidney tissue lysate
      Lane 3 : Human kidney tissue lysate

      Lysates/proteins at 40 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 19 kDa


      Exposure time: 20 seconds

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab198216? Please let us know so that we can cite the reference in this datasheet.

    ab198216 has been referenced in 6 publications.

    • Xiao Y  et al. Interleukin-33 Promotes REG3? Expression in Intestinal Epithelial Cells and Regulates Gut Microbiota. Cell Mol Gastroenterol Hepatol 8:21-36 (2019). PubMed: 30831322
    • Berkowitz L  et al. Mucosal Exposure to Cigarette Components Induces Intestinal Inflammation and Alters Antimicrobial Response in Mice. Front Immunol 10:2289 (2019). PubMed: 31608070
    • Miyata T  et al. CXCL12 expression in intrahepatic cholangiocarcinoma is associated with metastasis and poor prognosis. Cancer Sci 110:3197-3203 (2019). PubMed: 31361379
    • Peng W  et al. Elevated HuR in Pancreas Promotes a Pancreatitis-Like Inflammatory Microenvironment That Facilitates Tumor Development. Mol Cell Biol 38:N/A (2018). PubMed: 29133460
    • Wang J  et al. Aryl hydrocarbon receptor/IL-22/Stat3 signaling pathway is involved in the modulation of intestinal mucosa antimicrobial molecules by commensal microbiota in mice. Innate Immun 24:297-306 (2018). PubMed: 29976114
    • Chen L  et al. NLRP12 attenuates colon inflammation by maintaining colonic microbial diversity and promoting protective commensal bacterial growth. Nat Immunol 18:541-551 (2017). PubMed: 28288099

    Customer reviews and Q&As

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    Immunohistochemistry (Frozen sections) abreview for Anti-REG3G antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (rat intestine (ileon))
    Permeabilization
    No
    Specification
    rat intestine (ileon)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 27 2016

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