Anti-Rel B antibody (ab154957)
Key features and details
- Rabbit polyclonal to Rel B
- Suitable for: WB, IHC-P, ChIP, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Rel B antibody
See all Rel B primary antibodies -
Description
Rabbit polyclonal to Rel B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ChIP, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 354-528 of Human Rel B (Q01201).
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Positive control
- ChIP: THP-1 chromatin extract; WB: Jurkat, transfected HEK-293T, NIH 3T3 whole cell lysates; IHC-P: Human breast carcinoma tissue; IP: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
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IHC-P |
1/100 - 1/1000.
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ChIP |
Use at an assay dependent concentration.
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IP |
1/100 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 62 kDa. |
IHC-P
1/100 - 1/1000. |
ChIP
Use at an assay dependent concentration. |
IP
1/100 - 1/500. |
Target
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Function
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49. -
Sequence similarities
Contains 1 RHD (Rel-like) domain. -
Domain
Both N- and C-terminal domains are required for transcriptional activation. -
Post-translational
modificationsPhosphorylation at 'Thr-103' and 'Ser-573' is followed by proteasomal degradation. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton > centrosome. Co-localizes with NEK6 in the centrosome. - Information by UniProt
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Database links
- Entrez Gene: 5971 Human
- Entrez Gene: 19698 Mouse
- Omim: 604758 Human
- SwissProt: Q01201 Human
- SwissProt: Q04863 Mouse
- Unigene: 654402 Human
- Unigene: 1741 Mouse
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Alternative names
- I REL antibody
- I-Rel antibody
- IREL antibody
see all
Images
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Cross-linked ChIP was performed with THP-1 (human monocytic leukemia cell line) chromatin extract treated with LPS (1.0 μg/ml for 3 h) and 5 μg of either normal rabbit IgG or ab154957. The precipitated DNA was detected by PCR with primer set targeting to IL-1β promoter.
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All lanes : Anti-Rel B antibody (ab154957) at 1/10000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 62 kDa -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue staining Rel B at cytoplasm and nucleus with ab154957 at 1/500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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RelB antibody immunoprecipitates RelB protein in IP experiments.
IP Sample: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
A. Control with 2 μg of preimmune rabbit IgG
B. Immunoprecipitation of RelB protein by 2 μg of ab154957
7.5% SDS-PAGE
The immunoprecipitated RelB protein was detected by ab154957 at 1/1000 dilution, followed by anti-rabbit IgG antibody.
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All lanes : Anti-Rel B antibody (ab154957) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 62 kDa7.5% SDS-PAGE
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Anti-Rel B antibody (ab154957) at 1/10000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 62 kDa
7.5% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab154957 has been referenced in 3 publications.
- Wang H et al. Lack of bombesin receptor-activated protein attenuates bleomycin-induced pulmonary fibrosis in mice. Life Sci Alliance 5:N/A (2022). PubMed: 35820707
- Wang Z et al. miR-873-5p Inhibits Cell Migration and Invasion of Papillary Thyroid Cancer via Regulation of CXCL16. Onco Targets Ther 13:1037-1046 (2020). PubMed: 32099406
- He S et al. I-?B kinase-e knockout protects against angiotensin II induced aortic valve thickening in apolipoprotein E deficient mice. Biomed Pharmacother 109:1287-1295 (2019). PubMed: 30463808