Overview

  • Product name

    Anti-Rel B antibody [EP614Y]
    See all Rel B primary antibodies
  • Description

    Rabbit monoclonal [EP614Y] to Rel B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Rel B aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: Q01201

  • Positive control

    • Flow Cyt: Raji cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab212214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2500.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49.
  • Sequence similarities

    Contains 1 RHD (Rel-like) domain.
  • Domain

    Both N- and C-terminal domains are required for transcriptional activation.
  • Post-translational
    modifications

    Phosphorylation at 'Thr-103' and 'Ser-573' is followed by proteasomal degradation.
  • Cellular localization

    Nucleus. Cytoplasm > cytoskeleton > centrosome. Co-localizes with NEK6 in the centrosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • I REL antibody
    • I-Rel antibody
    • IREL antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
    • relB antibody
    • RELB_HUMAN antibody
    • Reticuloendotheliosis viral oncogene homolog B antibody
    • Transcription factor Rel B antibody
    • Transcription factor RelB antibody
    • v rel avian reticuloendotheliosis viral oncogene homolog B antibody
    • v rel reticuloendotheliosis viral oncogene homolog B antibody
    see all

Images

  • Overlay histogram showing Raji cells stained with ab212214 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab212214, 1/2500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 BP bandpass filter.

    This antibody gave a positive signal in Raji cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab212214 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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