Overview

  • Product name
    Anti-RELM alpha/Retnla antibody
    See all RELM alpha/Retnla primary antibodies
  • Description
    Rabbit polyclonal to RELM alpha/Retnla
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein corresponding to Mouse RELM alpha/Retnla.

  • General notes

     This product was previously labelled as RELM alpha

     

Properties

  • Form
    Lyophilised:Reconstitute in sterile water to 0.2 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab39626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
ELISA Use a concentration of 0.5 µg/ml.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa.

Target

  • Function
    Probable hormone.
  • Tissue specificity
    Highest levels in adipose tissue.
  • Sequence similarities
    Belongs to the resistin/FIZZ family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cysteine-rich secreted protein A12-gamma antibody
    • Cysteine-rich secreted protein FIZZ1 antibody
    • FIZZ1 antibody
    • Parasite-induced macrophage novel gene 1 protein antibody
    • RELMalpha antibody
    • Resistin like alpha antibody
    • Resistin-like alpha antibody
    • RETNA_MOUSE antibody
    • Retnla antibody
    see all

Images

  • ab39626 staining RELM alpha/Retnla in Mouse cecum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (4 ng/ml) overnight at 4°C. A Cy3-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody.

  • ab39626 at 4 ng/ml staining murine cecum tissue (with positive cells infiltrating the Lamina Propia of a Trichuris muris infected mouse) by IHC-P. The primary antibody was incubated overnight at 4˚C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit IgG.  Heat induced antigen retrieval with a 100mM Citric Acid was used.
  • ab39626 staining RELM alpha/Retnla in murine liver tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in paraformaldehyde and then blocked using 5% BSA for 30 minutes. Samples were then incubated with ab39626 at a 1/20 dilution for 1 hour. The secondary used was a biotinylated goat anti-rabbit IgG used at a 1/200 dilution.

    See Abreview

References

This product has been referenced in:
  • Yin Y  et al. The homing of human umbilical cord-derived mesenchymal stem cells and the subsequent modulation of macrophage polarization in type 2 diabetic mice. Int Immunopharmacol 60:235-245 (2018). IHC-Fr ; Mouse . Read more (PubMed: 29778021) »
  • Braun GS  et al. IL-6 Trans-Signaling Drives Murine Crescentic GN. J Am Soc Nephrol 27:132-42 (2016). Read more (PubMed: 26041841) »
See all 15 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

We have not suggested a positive control for ab39626. However, RELM alpha is predominantly found in adipose tissue therefore I could suggest Adipose (Mouse) Whole Cell Lysate - normal tissue (ab29417) as a positive control.

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Answer

Thank you very much for contacting us with your enquiry.

I apologize for the delay getting this SDS for you. There was an IT issue with the PDF on the website, but this has now been corrected. I have also attached the PDF version for you.

Please let me know if you have any further questions, or if there is anything else that we can do for you. Have a nice day.

Read More

Question
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheets and for the inconvenience this has caused. I have issued free of charge replacements. To check the status of the order please contact our Customer Service team and reference the order number.

Please note that these free of charge replacement vials are also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Perineal adipose tissue)
Permeabilization
No
Specification
Perineal adipose tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Mar 20 2012

Answer

Thank you for contacting us and your interest in our products.


As discussed over the phone ab8918isamouse monoclonaland canbe used with an anti-mouse IgG secondary antibody.


ab39626 and ab9969 are bothrabbit polyclonals andanti-rabbitIgG can be used in their detection.


If youdo find any non-specificitywith the anti-mouse secondary with your mouse sample (most likely a band at˜25 kDaand/or˜55 kDa)I would suggest looking at the followingprotocols:


https://www.abcam.com/index.html?pageconfig=resource&rid=11465

http://www.ihcworld.com/_protocols/general_IHC/immuno_mom.htm


Thesearefor IHC but should betransferable toWestern blotting. Alternatively I would suggest altering theprimary antibody to a non-mouseprimary.


I hope this information has beenof help. If you have any problems or require any further information please do not hesitate to ask.

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Answer

Thank you for contacting us. The complete datasheet for each product can be found online. For ab39626 it is: https://www.abcam.com/RELM-alpha-antibody-ab39626.html There is also a button to create a pdf-file. I have attached this file to this email. There are no specific protocols listed for this antibody in the protocols tab. But please let me know which application you are using and I can look into that. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jun 22 2011

Application
Western blot
Sample
Mouse Cell lysate - whole cell (bone marrow derived macrophages)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
40 µg
Treatment
DMSO and IL-4 10 ng/ml
Specification
bone marrow derived macrophages
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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