Overview

  • Product name

  • Description

    Rabbit polyclonal to RELM beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein corresponding to Mouse RELM beta.

  • Positive control

    • Mouse colon.

Properties

Applications

Our Abpromise guarantee covers the use of ab11429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 12 kDa).

Detects a band of approximately 18 kDa representing recombinant mouse RELM beta.

Target

  • Function

    Probable hormone.
  • Tissue specificity

    Expressed only in the gastrointestinal tract, particularly the colon.
  • Sequence similarities

    Belongs to the resistin/FIZZ family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 2 antibody
    • CCGR antibody
    • Colon and small intestine specific cysteine rich protein antibody
    • Colon and small intestine-specific cysteine-rich protein antibody
    • Colon carcinoma related gene protein antibody
    • Colon carcinoma-related gene protein antibody
    • Cysteine rich secreted A12 alpha like protein 1 antibody
    • Cysteine rich secreted protein A12 alpha like 1 antibody
    • Cysteine rich secreted protein FIZZ2 antibody
    • Cysteine-rich secreted protein A12-alpha-like 1 antibody
    • Cysteine-rich secreted protein FIZZ2 antibody
    • FIZZ1 antibody
    • FIZZ2 antibody
    • Found in inflammatory zone 1 antibody
    • HXCP2 antibody
    • RELM beta antibody
    • RELMb antibody
    • RELMbeta antibody
    • Resistin like beta antibody
    • Resistin like protein beta antibody
    • Resistin-like beta antibody
    • RETNB_HUMAN antibody
    • RETNL2 antibody
    • Retnlb antibody
    • XCP2 antibody
    see all

Images

  • ab11429 labelling RELM in Mouse colon tissue sections by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections).

  • ab11429 labelling RELM in Mouse colon tissue sections by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections).

References

This product has been referenced in:

  • Steel N  et al. TGFß-activation by dendritic cells drives Th17 induction and intestinal contractility and augments the expulsion of the parasite Trichinella spiralis in mice. PLoS Pathog 15:e1007657 (2019). Read more (PubMed: 30998782) »
  • Jiang Y  et al. TGF-ß1-induced SMAD2/3/4 activation promotes RELM-ß transcription to modulate the endothelium-mesenchymal transition in human endothelial cells. Int J Biochem Cell Biol 105:52-60 (2018). Read more (PubMed: 30120989) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for your phone call yesterday and for your patience while I have been in touch with the lab. Ab11429 has not been tested against other RELM proteins and it is unknown whether it will cross-react with the other forms. This polyclonal antibody was raised against full-length recombinant mouse RELM beta, so there will likely be epitopes all over the protein surface and if there are regions of homology with other RELM proteins there may be crossreactivity. If you are having any problems with the antibody or if you have concerns, please let me know and I will be happy to help you.

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Answer

Thank you for your enquiry. I am sorry to hear that you have been having problems with this antibody. I have read your technical questionaire and I have a few comments. You are using mouse intestine which is a positive control that we recommend. However, I would appreciate it if you could tell me the dilution factor that you have been applying the antibody at. We recommend the antibody is applied at a dilution of 1:100. Could you also tell me whether the permeabilization agent is triton? I am currently in correspondence with my colleagues in the lab to determine the protocol that was employed for the testing of this antibody by IHC-P. I will forward any information that becomes available.

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