Key features and details
- Rabbit polyclonal to RELM beta (Biotin)
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-RELM beta antibody (Biotin)
See all RELM beta primary antibodies
DescriptionRabbit polyclonal to RELM beta (Biotin)
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Mouse
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferConstituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa.|
|Sandwich ELISA||Use at an assay dependent concentration.|
Tissue specificityExpressed only in the gastrointestinal tract, particularly the colon.
Sequence similaritiesBelongs to the resistin/FIZZ family.
- Information by UniProt
- C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 2 antibody
- CCGR antibody
- Colon and small intestine specific cysteine rich protein antibody
To detect mouse RELM beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml.
When used in conjunction with compatible secondary reagents, the detection limit for recombinant mouse RELM beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse RELM beta, respectively.
Mouse RELMβ was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271225.
This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mouse RELMβ protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271225 has not yet been referenced specifically in any publications.