• Product name
  • Description
    Goat polyclonal to Renalase
  • Host species
  • Specificity
    ab31291 recognises renalase.
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:


    , corresponding to Internal sequence amino acids 224-234 of Human Renalase

  • Positive control
    • Human or mouse kidney lysate



Our Abpromise guarantee covers the use of ab31291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/32000.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-P Use a concentration of 2 - 4 µg/ml.

In paraffin embedded Human Kidney shows vesicular staining in the epithelial cells of the CT.


  • Function
    Probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. Degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. Lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis (By similarity). According to PubMed:17385068, is unlikely that renalase has physiologically relevant catecholamine-oxidizing activity.
  • Tissue specificity
    Secreted into the blood by the kidney. Highly expressed in the kidney, expressed at lower level in heart, skeletal muscle and small intestine. Its plasma concentration is markedly reduced in patients with end-stage renal disease, as compared with healthy subjects.
  • Sequence similarities
    Belongs to the renalase family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • 6530404N21Rik antibody
    • AI452315 antibody
    • AW060440 antibody
    • C10orf59 antibody
    • Chromosome 10 open reading frame 59 antibody
    • FLJ11218 antibody
    • HGNC:25641 antibody
    • Hypothetical protein LOC55328 antibody
    • MAO C antibody
    • MAO-C antibody
    • mMAO C antibody
    • Monoamine oxidase C antibody
    • Monoamine oxidase-C antibody
    • Renalase antibody
    • Renalase FAD dependent amine oxidase antibody
    • RNLS antibody
    • RNLS_HUMAN antibody
    see all


  • In paraffin embedded Human Kidney using ab31291 it shows vesicular staining in the epithelial cells of the CT

  • Anti-Renalase antibody (ab31291) at 1 µg/ml (1 hour incubation) + Human Kidney lysate (35µg protein in RIPA buffer)

    Developed using the ECL technique.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

  • ab31291 (3µg/ml) staining Human Kidney by IHC-P. Microwaved antigen retrieval with citrate buffer (pH 6), HRP-staining.


This product has been referenced in:
  • Hollander L  et al. Renalase Expression by Melanoma and Tumor-Associated Macrophages Promotes Tumor Growth through a STAT3-Mediated Mechanism. Cancer Res 76:3884-94 (2016). Read more (PubMed: 27197188) »
  • Guo X  et al. Inhibition of renalase expression and signaling has antitumor activity in pancreatic cancer. Sci Rep 6:22996 (2016). Read more (PubMed: 26972355) »

See all 6 Publications for this product

Customer reviews and Q&As

Thanks for your reply.

I think it will be very important, based on my knowledge of the situation, to get some specific details from the authors of the paper whose experiment you wish to reproduce. I suggest sending another email within a day ...

Read More

Regarding your enquiry, I have spoken with my colleague John who you spoke with on August 9. He mentioned advising you to contact the authors of the study you are attempting to duplicate. I just wanted to know if you have heard from them yet ...

Read More

Thank you for your email.

Yes, with the peptide you can make the standard curve.

When using unknown samples, you would just coat the well with the samples, but not the peptide. The peptide would be in separate wells. Also, try seve...

Read More

Thank you for contacting us.

ELISA assay is used for determining the activity of the antibody by applying serially diluted antibody to micro-wells that are coated with the immunizing peptide. Please find below the protocol that was used, inc...

Read More

Thank you for your enquiry. Unfortunately we do not provide the standard protein. This product was tested in peptide-ELISA, with the immunizing peptide bound to the plate. I am sorry I can't be of more assistance in this occasion. If there is a...

Read More

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab31291 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the ...

Read More

Thank you for your enquiry. I have copied the ELISA protocol below: Peptide ELISA - Plates coated with immunising peptide at 1ug/ml, 4C overnight - Plates blocked blocked in 2.5% skimmed milk in TBS-T (TBS + 0.05%Tween-20) for 1 hr at room te...

Read More


Sign up