Overview

  • Product name

    Anti-Renalase antibody [EPR4212(2)]
    See all Renalase primary antibodies
  • Description

    Rabbit monoclonal [EPR4212(2)] to Renalase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Renalase aa 50-150. The exact sequence is proprietary.
    Database link: Q5VYX0

  • Positive control

    • Fetal heart, fetal kidney, Human skeletal muscle, Human plasma, A673 and 293T lysates; Human heart and Human kidney tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab178700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. Degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. Lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis (By similarity). According to PubMed:17385068, is unlikely that renalase has physiologically relevant catecholamine-oxidizing activity.
    • Tissue specificity

      Secreted into the blood by the kidney. Highly expressed in the kidney, expressed at lower level in heart, skeletal muscle and small intestine. Its plasma concentration is markedly reduced in patients with end-stage renal disease, as compared with healthy subjects.
    • Sequence similarities

      Belongs to the renalase family.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • 6530404N21Rik antibody
      • AI452315 antibody
      • AW060440 antibody
      • C10orf59 antibody
      • Chromosome 10 open reading frame 59 antibody
      • FLJ11218 antibody
      • HGNC:25641 antibody
      • Hypothetical protein LOC55328 antibody
      • MAO C antibody
      • MAO-C antibody
      • mMAO C antibody
      • Monoamine oxidase C antibody
      • Monoamine oxidase-C antibody
      • Renalase antibody
      • Renalase FAD dependent amine oxidase antibody
      • RNLS antibody
      • RNLS_HUMAN antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Renalase with ab178700 at 1/50.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Human heart tissue labeling Renalase with ab178700 at 1/50.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • All lanes : Anti-Renalase antibody [EPR4212(2)] (ab178700) at 1/1000 dilution

      Lane 1 : Fetal heart lysate
      Lane 2 : Fetal kidney lysate
      Lane 3 : Human skeletal muscle lysate
      Lane 4 : Human plasma lysate
      Lane 5 : A673 lysate
      Lane 6 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 38 kDa
      Observed band size: 42 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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