Overview

Description

  • Nature
    Synthetic
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      C-VSIDNKKRNI
    • Amino acids
      224 to 234

Associated products

Specifications

Our Abpromise guarantee covers the use of ab45730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Renalase antibody (ab31291)

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Info

  • Alternative names
    • 6530404N21Rik
    • AI452315
    • AW060440
    • C10orf59
    • Chromosome 10 open reading frame 59
    • FLJ11218
    • HGNC:25641
    • Hypothetical protein LOC55328
    • MAO C
    • MAO-C
    • mMAO C
    • Monoamine oxidase C
    • Monoamine oxidase-C
    • Renalase
    • Renalase FAD dependent amine oxidase
    • RNLS
    • RNLS_HUMAN
    see all
  • Function
    Probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. Degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. Lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis (By similarity). According to PubMed:17385068, is unlikely that renalase has physiologically relevant catecholamine-oxidizing activity.
  • Tissue specificity
    Secreted into the blood by the kidney. Highly expressed in the kidney, expressed at lower level in heart, skeletal muscle and small intestine. Its plasma concentration is markedly reduced in patients with end-stage renal disease, as compared with healthy subjects.
  • Sequence similarities
    Belongs to the renalase family.
  • Cellular localization
    Secreted.
  • Information by UniProt

References

ab45730 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for contacting us.

As a suitable positive control for an anti-human renalase antibody, I would recommend the following product catalogue reference number ab134535 : Renalase protein (His tag), https://www.abcam.com/ab134535.

The peptide ab45730 is only 10 amino acids long and can be used as a specific blocking peptide for the Abcam Renalase antibody catalogue reference number ab31291.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thanks for your reply.

I think it will be very important, based on my knowledge of the situation, to get some specific details from the authors of the paper whose experiment you wish to reproduce. I suggest sending another email within a day or 2 if you do not hear from them.

I look forward to hearing how things go.

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Answer

Thank you for your email.

Yes, with the peptide you can make the standard curve.

When using unknown samples, you would just coat the well with the samples, but not the peptide. The peptide would be in separate wells. Also, try several amounts of thesample as to determine which will give you a good signal.So, either peptide or sample are coated and then you would use the antibody to detect peptide or sample. Subsequently, a secondary antibody (HRP or AP conjugated) will be used to obtain the signal/read out.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank you for contacting us.

ELISA assay is used for determining the activity of the antibody by applying serially diluted antibody to micro-wells that are coated with the immunizing peptide. Please find below the protocol that was used, including the peptide amount:

Wells are coated with the immunizing peptide and serially diluted antibodies are applied and measured.



Coat 0.1ug peptide in 100ul PBS per well on high binding ELISA plates overnight at 4C.
Wash 3 times with 300ul PBS per well.
Block with PBS/0.1%(v/v) Tween-20 (PBST) containing 3%(w/v) skimmed milk (blocking buffer) for 1h at 37C
Wash 3 times with PBST
Make serially dilutions of antibody in blocking buffer and apply 100ul per well.
Incubate 1h at 37C with the plates covered
Wash three times with 300ul PBST
Apply secondary antibody (AP-conjugate) at appropriate dilution in blocking buffer, 100ul per well
Wash four times in 300ul PBST
Wash 2 times in 300ul PBS
Add 50ul per well p-nitro phenyl phosphate (pNPP) from Sigma and incubate for 30min at 30C
Stop the reaction with 50ul 1M NaOH.
Read at 405nm



Phosphate buffered saline (PBS): 20mM Sodium phosphate, pH7.4 in 150mM NaCl.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank you for contacting Abcam about ab45730. I have contacted the lab to clarify what liquid the peptide, ab45730, is in and they have confirmed that unless otherwise stated, the peptide is in H2O. If there is anything else I can help you with, please let me know.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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