Overview

  • Product name

    Anti-Renilla Luciferase antibody
    See all Renilla Luciferase primary antibodies
  • Description

    Rabbit polyclonal to Renilla Luciferase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IP, Neutralisingmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Recombinant full length protein corresponding to Renilla Luciferase aa 1-311. (Expressed in E. coli).
    Database link: P27652

  • Positive control

    • Renilla luciferase transfected 293 whole cell lysate; Recombinant Renilla luciferase; pCMV-Renilla luciferase transfected 293 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 69% PBS, 30% Glycerol, 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/4000.
WB 1/2000 - 1/4000. Predicted molecular weight: 36 kDa.Can be blocked with Recombinant Renilla Luciferase protein (Tagged) (ab226271).
IP Use at 25 µg/mg of lysate.
Neutralising Use at an assay dependent concentration.

Target

  • Relevance

    The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly.
  • Database links

    • Alternative names

      • Renilla luciferin 2 monooxygenase antibody
      • Renilla type luciferase antibody

    Images

    • All lanes : Anti-Renilla Luciferase antibody (ab187338) at 1/4000 dilution

      Lane 1 : Non-transfected 293 whole cell lysate
      Lane 2 : Renilla Luciferase transfected 293 whole cell lysate
      Lane 3 : Red Firefly Luciferase transfected 293 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/500 dilution

      Developed using the ECL technique.

      Predicted band size: 36 kDa

    • All lanes : Anti-Renilla Luciferase antibody (ab187338) at 1/4000 dilution

      Lane 1 : Non-transfected 293 whole cell lysate
      Lane 2 : Renilla Luciferase transfected 293 whole cell lysate
      Lane 3 : Not Green-shifted Renilla reniformis Luciferase transfected 293 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/500 dilution

      Developed using the ECL technique.

      Predicted band size: 36 kDa

    • All lanes : Anti-Renilla Luciferase antibody (ab187338) at 1/4000 dilution

      Lane 1 : Full length recombinant Renilla Luciferase at 100 µg
      Lane 2 : Full length recombinant Renilla Luciferase at 50 µg
      Lane 3 : Full length recombinant Renilla Luciferase at 25 µg
      Lane 4 : Full length recombinant Renilla Luciferase at 10 µg

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/500 dilution

      Developed using the ECL technique.

      Predicted band size: 36 kDa

    • Immunofluorescent analysis of Renilla Luciferase (from the Sea pansy Renilla reniformis) in untransfected (left panel) and pCMV-Renilla Luciferase transfected (right panel) 293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were probed with ab187338 at 1/1000 dilution for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody 1/400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. 

    • Immunoprecipitation of Renilla Luciferase (from the Sea pansy Renilla reniformis) was performed using whole cell lysates from untransfected and Renilla Luciferase or Red Firefly luciferase transfected 293 cell lines. Antigen-antibody complexes were formed by incubating 200ug of lysate with 5ug of ab187338 overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted. Whole cell lysates from untransfected and transfected whole cell lysates (2ug) were used as controls. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween-20 for 1 hour. The membrane was probed with ab187338 at a dilution of 1/4000 overnight rotating at 4°C.

       

      Lane 1: Non-transfected 293 whole cell lysate

      Lane 2: Renilla Luciferase IP from non-transfected 293 whole cell lysate

      Lane 3: Renilla Luciferase transfected 293 whole cell lysate

      Lane 4: Renilla Luciferase IP from Renilla Luciferase transfected 293 whole cell lysate

      Lane 5: Red Firefly Luciferase transfected 293 whole cell lysate

      Lane 6: Renilla Luciferase IP from Red Firefly Luciferase transfected 293 whole cell lysate

    • Neutralizing effect of Renilla luciferase antibody on the activity of purified recombinant Renilla luciferase (from the Sea pansy Renilla reniformis) using a Renilla Luciferase Assay Kit. 1ng of recombinant enzyme were incubated with the indicated amounts of ab187338 for at least 30 min. Enzyme activity (RLU) was detected. Average RUI values from 10 timepoints per concentration of 4 replicates +/- standard deviation are shown.

    References

    This product has been referenced in:

    • Bunda S  et al. CIC protein instability contributes to tumorigenesis in glioblastoma. Nat Commun 10:661 (2019). Read more (PubMed: 30737375) »
    • Yanik M  et al. Development of a Reporter System to Explore MMEJ in the Context of Replacing Large Genomic Fragments. Mol Ther Nucleic Acids 11:407-415 (2018). Read more (PubMed: 29858075) »
    See all 2 Publications for this product

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