Recombinant
RabMAb

Recombinant Anti-Renilla Luciferase antibody [EPR17791] (ab185925)

Overview

  • Product name
    Anti-Renilla Luciferase antibody [EPR17791]
    See all Renilla Luciferase primary antibodies
  • Description
    Rabbit monoclonal [EPR17791] to Renilla Luciferase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Other species
    Does not react with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Renilla Luciferase aa 1-100. The exact sequence is proprietary. Renilla reniformis (Sea pansy).
    Database link: P27652

  • Positive control
    • WB: Renilla Luciferase transfected HEK293T whole cell lysate. ICC/IF: Renilla Luciferase transfected HEK293T cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR17791
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ICC/IF 1/1000.

Target

  • Relevance
    The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly.
  • Database links
    • Alternative names
      • Renilla luciferin 2 monooxygenase antibody
      • Renilla type luciferase antibody

    Images

    • All lanes : Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

      Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate
      Lane 2 : Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 36 kDa
      Observed band size: 36 kDa


      Exposure time: 15 seconds
    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Renilla Luciferase, labeling Renilla Luciferase with ab185925 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK-293T cells transfected with Renilla Luciferase is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows;
      -ve control 1: ab185925 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    • All lanes : Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

      Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate
      Lane 2 : Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate
      Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) lysate
      Lane 4 : MCF7 (Human breast adenocarcinoma cell line) lysate
      Lane 5 : Jurkat (Human T cell leukemia cells from peripheral blood) lysate
      Lane 6 : SH-SY5Y (Human neuroblastoma from bone marrow cells) lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 36 kDa
      Observed band size: 36 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

      Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate at 20 µg
      Lane 2 : Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg
      Lane 3 : Human fetal brain lysate at 10 µg
      Lane 4 : Human fetal heart lysate at 10 µg
      Lane 5 : Human fetal kidney lysate at 10 µg
      Lane 6 : Human fetal spleen lysate at 10 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 36 kDa
      Observed band size: 36 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

      Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate
      Lane 2 : Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate
      Lane 3 : Mouse brain lysate
      Lane 4 : Mouse heart lysate
      Lane 5 : Mouse kidney lysate
      Lane 6 : Mouse spleen lysate
      Lane 7 : Rat brain lysate
      Lane 8 : Rat heart lysate
      Lane 9 : Rat kidney lysate
      Lane 10 : Rat spleen lysate
      Lane 11 : C6 (Rat glial tumor cells) lysate
      Lane 12 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate
      Lane 13 : PC-12 (Rat adrenal gland pheochromocytoma) lysate
      Lane 14 : NIH/3T3 (Mouse embyro fibroblast cells) lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 36 kDa
      Observed band size: 36 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    References

    This product has been referenced in:
    • Zhou Y  et al. Disruption of TWIST1 translation by 5' UTR variants in Saethre-Chotzen syndrome. Hum Mutat 39:1360-1365 (2018). Read more (PubMed: 30040876) »
    • Braid LR  et al. Intramuscular administration potentiates extended dwell time of mesenchymal stromal cells compared to other routes. Cytotherapy 20:232-244 (2018). Read more (PubMed: 29167063) »
    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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