Recombinant
RabMAb

Anti-Renilla Luciferase antibody [EPR17791] (Alexa Fluor® 488) (ab216113)

Overview

  • Product name
    Anti-Renilla Luciferase antibody [EPR17791] (Alexa Fluor® 488)
    See all Renilla Luciferase primary antibodies
  • Description
    Rabbit monoclonal [EPR17791] to Renilla Luciferase (Alexa Fluor® 488)
  • Host species
    Rabbit
  • Conjugation
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Other species
  • Immunogen

    Synthetic peptide within Renilla Luciferase aa 1-100. The exact sequence is proprietary. Renilla reniformis (Sea pansy).
    Database link: P27652

  • Positive control
    • ICC/IF: HEK293T cells transfected with RFP-tagged Renilla Luciferase
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer
    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR17791
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab216113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.

This product gave a positive signal in HEK293T cells transfected with RFP-tagged Renilla Luciferase fixed with 4% formaldehyde (10 min)

Target

  • Relevance
    The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly.
  • Database links
    • Alternative names
      • Renilla luciferin 2 monooxygenase antibody
      • Renilla type luciferase antibody

    Images

    • ab216113 staining Firefly Luciferase in HEK293T cells transfected with RFP-tagged Renilla Luciferase expression construct or RFP-only-tagged expression construct. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.

      The cells were then incubated overnight at +4°C with ab214950 at 1/200 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue) and RFP is shown in red.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    References

    ab216113 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab216113.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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