Recombinant
RabMAb

Anti-Renilla Luciferase antibody [EPR17792] (ab185926)

Overview

  • Product name
    Anti-Renilla Luciferase antibody [EPR17792]
    See all Renilla Luciferase primary antibodies
  • Description
    Rabbit monoclonal [EPR17792] to Renilla Luciferase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Other species
    Does not react with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Renilla Luciferase aa 150-250. The exact sequence is proprietary. Renilla reniformis (Sea pansy).
    Database link: P27652

  • Positive control
    • WB: Renilla Luciferase transfected HEK293T whole cell lysate. ICC/IF: Renilla Luciferase transfected HEK293T cells. Flow Cyt: Renilla Luciferase transfected HEK293T cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR17792
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
WB 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance
    The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly.
  • Database links
    • Alternative names
      • Renilla luciferin 2 monooxygenase antibody
      • Renilla type luciferase antibody

    Images

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Renilla Luciferase, labeling Renilla Luciferase with ab185926 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK293T cells transfected with Renilla Luciferase is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows;
      1. ab185926 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    • Flow Cytometry analysis of 293T (Human epithelial cell line from embryonic kidney) transfected with GFP tagged Renilla Luciferase cells labeled with purified ab185926 at 1/70 dilution (10µg/ml, Right panel) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 647)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Left panel) was used as the isotype control.

    References

    ab185926 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse ESCs)
    Permeabilization
    Yes - Triton
    Specification
    mouse ESCs
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3%
    Fixative
    Formaldehyde
    Username

    Diego Rodriguez Terrones

    Verified customer

    Submitted Sep 01 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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