Recombinant
RabMAb

Recombinant Anti-Renilla Luciferase antibody [EPR17792] (Phycoerythrin) (ab225340)

Overview

  • Product name

    Anti-Renilla Luciferase antibody [EPR17792] (Phycoerythrin)
    See all Renilla Luciferase primary antibodies
  • Description

    Rabbit monoclonal [EPR17792] to Renilla Luciferase (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity


    Predicted to work with: Other speciesDoes not react with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Renilla Luciferase aa 150-250. The exact sequence is proprietary.
    Database link: P27652

  • Positive control

    • Flow Cyt: 293T cells transfected with RFP tagged Renilla Luciferase.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17792
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab225340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Relevance

    The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly.
  • Database links

    • Alternative names

      • Renilla luciferin 2 monooxygenase antibody
      • Renilla type luciferase antibody

    Images

    • Overlay histogram showing 293T transfected with RFP tagged Renilla Luciferase stained with ab225340 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225340, 1/50 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 20 mW blue solid-state laser (488nm) and 585/42 bandpass filter.

    References

    ab225340 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab225340.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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