Anti-Renin antibody (ab180608)

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Western blot - Anti-Renin antibody (ab180608)

    Key features and details

    • Rabbit polyclonal to Renin
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-Renin antibody
      See all Renin primary antibodies

    • Description

      Rabbit polyclonal to Renin

    • Host species

      Rabbit

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Recombinant full length protein corresponding to Human Renin aa 67-406. Mature protein.
      Sequence:

      LTLGNTTSSVILTNYMDTQYYGEIGIGTPPQTFKVVFDTGSSNVWVPSSK CSRLYTACVYHKLFDASDSSSYKHNGTELTLRYSTGTVSGFLSQDIITVG GITVTQMFGEVTEMPALPFMLAEFDGVVGMGFIEQAIGRVTPIFDNIISQ GVLKEDVFSFYYNRDSENSQSLGGQIVLGGSDPQHYEGNFHYINLIKTGV WQIQMKGVSVGSSTLLCEDGCLALVDTGASYISGSTSSIEKLMEALGAKK RLFDYVVKCNEGPTLPDISFHLGGKEYTLTSADYVFQESYSSKKLCTLAI HAMDIPPPTGPTWALGATFIRKFYTEFDRRNNRIGFALAR


      Database link: P00797
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • K562, 293T and HepG2 cell extracts.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab180608 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Chimpanzee
    Cynomolgus monkey
    Rhesus monkey
    Application Abreviews Notes
    WB (1)
    1/500 - 1/2000. Predicted molecular weight: 45 kDa.
    Notes
    WB
    1/500 - 1/2000. Predicted molecular weight: 45 kDa.

    Target

    • Function

      Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

    • Involvement in disease

      Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
      Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia.

    • Sequence similarities

      Belongs to the peptidase A1 family.

    • Cellular localization

      Secreted. Membrane. Associated to membranes via binding to ATP6AP2.
    • Information by UniProt

    • Database links

      • Alternative names

        • Angiotensin forming enzyme antibody
        • Angiotensin forming enzyme precursor antibody
        • Angiotensinogenase antibody
        • Angiotensinogenase precursor antibody
        • FLJ10761 antibody
        • HNFJ2 antibody
        • REN antibody
        • Ren1 antibody
        • RENI_HUMAN antibody
        • Renin antibody
        • Renin precursor renal antibody
        see all

      Images

      • Western blot - Anti-Renin antibody (ab180608)
        Western blot - Anti-Renin antibody (ab180608)
        All lanes : Anti-Renin antibody (ab180608) at 1/500 dilution

        Lane 1 : K562 cell extract
        Lane 2 : 293T cell extract
        Lane 3 : HepG2 cell extract

        Predicted band size: 45 kDa

      References (3)

      Publishing research using ab180608? Please let us know so that we can cite the reference in this datasheet.

      ab180608 has been referenced in 3 publications.

      • Lóry V  et al. Obesity and aging affects skeletal muscle renin-angiotensin system and myosin heavy chain proportions in pre-diabetic Zucker rats. J Physiol Biochem 75:351-365 (2019). PubMed: 31197649
      • Ding L  et al. Elemene inhibits osteosarcoma growth by suppressing the renin-angiotensin system signaling pathway. Mol Med Rep 17:1022-1030 (2018). PubMed: 29115494
      • Yan K & Shen Y Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system. Mol Med Rep 16:3965-3973 (2017). PubMed: 28765966

      Customer reviews and Q&As

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      Western blot abreview for Anti-Renin antibody

       Poor
      Abreviews
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (kidney cortex)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      15 µg
      Specification
      kidney cortex
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

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      Verified customer

      Submitted Apr 22 2019

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