Recombinant
RabMAb

Recombinant Anti-Renin antibody [EPR20693] (ab212197)

Overview

  • Product name

    Anti-Renin antibody [EPR20693]
    See all Renin primary antibodies
  • Description

    Rabbit monoclonal [EPR20693] to Renin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Renin aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: P00797

  • Positive control

    • IHC-P: Human and mouse kidney tissues. IHC-Fr: Mouse and rat kidney tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab212197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000.

(for human); Use at 1/5000 dilution (for mouse).

This antibody is not recommended for rat species in IHC application. In our hands this antibody showed non-specific staining on rat kidney tissue sections

IHC-Fr 1/6000.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
    • Involvement in disease

      Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
      Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia.
    • Sequence similarities

      Belongs to the peptidase A1 family.
    • Cellular localization

      Secreted. Membrane. Associated to membranes via binding to ATP6AP2.
    • Information by UniProt
    • Database links

    • Alternative names

      • Angiotensin forming enzyme antibody
      • Angiotensin forming enzyme precursor antibody
      • Angiotensinogenase antibody
      • Angiotensinogenase precursor antibody
      • FLJ10761 antibody
      • HNFJ2 antibody
      • REN antibody
      • Ren1 antibody
      • RENI_HUMAN antibody
      • Renin antibody
      • Renin precursor renal antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Renin with ab212197 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on juxtaglomerular cells of human kidney (PMID: 1898348). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Heat mediated antigen retrieval performed using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Renin with ab212197  at 1/6000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in juxtaglomerular cells of mouse kidney tissue section (PMID: 27080979).

      The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      Heat mediated antigen retrieval performed using Tris-EDTA buffer (pH9.0) (ab94681).

    • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Renin with ab212197 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on juxtaglomerular cells of mouse kidney (PMID: 1898348). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Heat mediated antigen retrieval performed using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Renin with ab212197  at 1/6000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in juxtaglomerular cells of rat kidney tissue section (PMID: 27080979).

      The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      Heat mediated antigen retrieval performed using Tris-EDTA buffer (pH9.0) (ab94681).

    References

    ab212197 has not yet been referenced specifically in any publications.

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