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RecombinantRabMAb

Recombinant Anti-Renin antibody [EPR20693] (ab212197)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (8) Submit a question References (1)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (ab212197)
  • Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (ab212197)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (ab212197)
  • Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (ab212197)
  • Anti-Renin antibody [EPR20693] (ab212197)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20693] to Renin
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
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Recombinant Human Renin protein (ab159326)

View more associated products

Overview

  • Product name

    Anti-Renin antibody [EPR20693]
    See all Renin primary antibodies
  • Description

    Rabbit monoclonal [EPR20693] to Renin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human and mouse kidney tissues. IHC-Fr: Mouse and rat kidney tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20693
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Other
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals

Associated products

  • Alternative Versions

    • Anti-Renin antibody [EPR20693] - BSA and Azide free (ab225743)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Renin protein (ab159326)
  • Related Products

    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab212197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (4)
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

(for human); Use at 1/5000 dilution (for mouse).

This antibody is not recommended for rat species in IHC application. In our hands this antibody showed non-specific staining on rat kidney tissue sections

IHC-Fr
1/6000.
Notes
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

(for human); Use at 1/5000 dilution (for mouse).

This antibody is not recommended for rat species in IHC application. In our hands this antibody showed non-specific staining on rat kidney tissue sections

IHC-Fr
1/6000.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
    • Involvement in disease

      Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
      Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia.
    • Sequence similarities

      Belongs to the peptidase A1 family.
    • Cellular localization

      Secreted. Membrane. Associated to membranes via binding to ATP6AP2.
    • Target information above from: UniProt accession P00797 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5972 Human
      • Entrez Gene: 19701 Mouse
      • Entrez Gene: 19702 Mouse
      • Entrez Gene: 24715 Rat
      • Omim: 179820 Human
      • SwissProt: P00797 Human
      • SwissProt: P00796 Mouse
      • SwissProt: P06281 Mouse
      • SwissProt: P08424 Rat
      • Unigene: 3210 Human
      • Unigene: 9831 Rat
      see all
    • Alternative names

      • Angiotensin forming enzyme antibody
      • Angiotensin forming enzyme precursor antibody
      • Angiotensinogenase antibody
      • Angiotensinogenase precursor antibody
      • FLJ10761 antibody
      • HNFJ2 antibody
      • REN antibody
      • Ren1 antibody
      • RENI_HUMAN antibody
      • Renin antibody
      • Renin precursor renal antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (ab212197)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (ab212197)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Renin with ab212197 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on juxtaglomerular cells of human kidney (PMID: 1898348). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Heat mediated antigen retrieval performed using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (ab212197)
      Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (ab212197)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Renin with ab212197  at 1/6000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in juxtaglomerular cells of mouse kidney tissue section (PMID: 27080979).

      The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      Heat mediated antigen retrieval performed using Tris-EDTA buffer (pH9.0) (ab94681).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (ab212197)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (ab212197)

      Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Renin with ab212197 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on juxtaglomerular cells of mouse kidney (PMID: 1898348). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Heat mediated antigen retrieval performed using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (ab212197)
      Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (ab212197)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Renin with ab212197  at 1/6000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in juxtaglomerular cells of rat kidney tissue section (PMID: 27080979).

      The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      Heat mediated antigen retrieval performed using Tris-EDTA buffer (pH9.0) (ab94681).

    • Anti-Renin antibody [EPR20693] (ab212197)
      Anti-Renin antibody [EPR20693] (ab212197)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab212197? Please let us know so that we can cite the reference in this datasheet.

    ab212197 has been referenced in 1 publication.

    • Kumar Gupta A  et al. Asynchronous mixing of kidney progenitor cells potentiates nephrogenesis in organoids. Commun Biol 3:231 (2020). PubMed: 32393756

    Customer reviews and Q&As

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    1-8 of 8 Abreviews

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Renin antibody [EPR20693]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Pig Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 29 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Renin antibody [EPR20693]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 12 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Renin antibody [EPR20693]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 25 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Renin antibody [EPR20693]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 25 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Renin antibody [EPR20693]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Kidney H9 Organoids)
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney H9 Organoids
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 06 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Renin antibody [EPR20693]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    kidney
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 24 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Renin antibody [EPR20693]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 24 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Renin antibody [EPR20693]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    kidney
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 24 2019

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