Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Kidney H9 Organoids)
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney H9 Organoids
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Sea Block
Dilution
1/1000
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
The development of juxtaglomerular cells in this day 23 kidney organoid sample is evident by staining with the Anti-Renin antibody which is expressed in pseudo colored Green. The nuclei are stained Blue with Hoechst. Fluorescent visualization using the Alexa647 fluorophore results in a true background free signal.
Abcam response
We would like to thank the reviewer for submitting this AbReview for ab212197. We are pleased that this antibody worked well for them in ICC/IF, an application that has not been tested before, and we appreciate the time and effort that the reviewer has put into testing this antibody in this application. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Feb 06 2020