Recombinant
RabMAb

Recombinant Anti-Renin antibody [EPR20693] - BSA and Azide free (ab225743)

Overview

  • Product name

    Anti-Renin antibody [EPR20693] - BSA and Azide free
    See all Renin primary antibodies
  • Description

    Rabbit monoclonal [EPR20693] to Renin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Renin aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: P00797

  • Positive control

    • IHC-P: Human kidney tissue.
  • General notes

    Ab225743 is the carrier-free version of ab212197. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab225743 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.??

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

This antibody is not recommended for rat species in IHC application. In our hands this antibody showed non-specific staining on rat kidney tissue sections

Target

  • Function

    Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
  • Involvement in disease

    Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
    Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia.
  • Sequence similarities

    Belongs to the peptidase A1 family.
  • Cellular localization

    Secreted. Membrane. Associated to membranes via binding to ATP6AP2.
  • Information by UniProt
  • Database links

  • Alternative names

    • Angiotensin forming enzyme antibody
    • Angiotensin forming enzyme precursor antibody
    • Angiotensinogenase antibody
    • Angiotensinogenase precursor antibody
    • FLJ10761 antibody
    • HNFJ2 antibody
    • REN antibody
    • Ren1 antibody
    • RENI_HUMAN antibody
    • Renin antibody
    • Renin precursor renal antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Renin with ab212197 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on juxtaglomerular cells of mouse kidney (PMID: 1898348). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval performed using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212197).

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Renin with ab212197  at 1/6000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in juxtaglomerular cells of mouse kidney tissue section (PMID: 27080979).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Heat mediated antigen retrieval performed using Tris-EDTA buffer (pH9.0) (ab94681).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212197).

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Renin with ab212197  at 1/6000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in juxtaglomerular cells of rat kidney tissue section (PMID: 27080979).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Heat mediated antigen retrieval performed using Tris-EDTA buffer (pH9.0) (ab94681).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212197).

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Renin with ab212197 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on juxtaglomerular cells of human kidney (PMID: 1898348). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval performed using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212197).

References

ab225743 has not yet been referenced specifically in any publications.

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