Product nameAnti-Renin antibody [EPR6433]
See all Renin primary antibodies
DescriptionRabbit monoclonal [EPR6433] to Renin
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human Renin aa 50-150. The exact sequence is proprietary.
Database link: P00797
- K562, 293T, and HepG2 whole cell lysate (ab7900). K562 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Dissociation constant (KD)KD = 1.70 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab125012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRenin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Involvement in diseaseDefects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia.
Sequence similaritiesBelongs to the peptidase A1 family.
Cellular localizationSecreted. Membrane. Associated to membranes via binding to ATP6AP2.
- Information by UniProt
- Angiotensin forming enzyme antibody
- Angiotensin forming enzyme precursor antibody
- Angiotensinogenase antibody
All lanes : Anti-Renin antibody [EPR6433] (ab125012) at 1/1000 dilution
Lane 1 : K562 lysate
Lane 2 : 293T lysate
Lane 3 : HepG2 lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Flow cytometric analysis of permeabilized K562 cells using ab125012 at 1/10 shown in red or a rabbit IgG negative control shown in green.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab125012 has not yet been referenced specifically in any publications.