Overview

  • Product name

    Renin Assay Kit (Fluorometric)
    See all Renin kits
  • Detection method

    Fluorescent
  • Sample type

    Cell Lysate
  • Assay type

    Quantitative
  • Sensitivity

    10 ng/ml
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Renin Assay Kit (Fluorometric) (ab138875) provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a TF3/TQ3 FRET peptide. In the FRET peptide the fluorescence of TF3 is quenched by TQ3. Upon cleavage into two separate fragments by renin, the fluorescence of TF3 is recovered, the fluorescent signal can be easily monitored by a fluorescence microplate reader at Ex/Em = 540/590 nm. This assay is about fifty fold more sensitive than an EDANS/DABCYL-based assay.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Assay Buffer 1 x 10ml
    Renin Red Substrate (100x) 1 x 50µl
    Renin Standard 1 x 25µl
  • Research areas

  • Function

    Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
  • Involvement in disease

    Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
    Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia.
  • Sequence similarities

    Belongs to the peptidase A1 family.
  • Cellular localization

    Secreted. Membrane. Associated to membranes via binding to ATP6AP2.
  • Information by UniProt
  • Alternative names

    • angiotensin-forming enzyme
    • Angiotensinogenase
    • HNFJ2
    • REN
    • RENI_HUMAN
    • Renin
    • renin precursor, renal
    see all

Images

  • Renin dose response was measured with ab138875 in a 96-well black solid plate using a fluorescence microplate reader. As low as 10 ng /mL Renin was detected with 60 minutes incubation in 37°C

Protocols

References

This product has been referenced in:

  • Takiyama Y  et al. Impacts of Diabetes and an SGLT2 Inhibitor on the Glomerular Number and Volume in db/db Mice, as Estimated by Synchrotron Radiation Micro-CT at SPring-8. EBioMedicine 36:329-346 (2018). Read more (PubMed: 30322799) »
  • Messaoudi S  et al. Endothelial Gata5 transcription factor regulates blood pressure. Nat Commun 6:8835 (2015). Functional Studies ; Mouse . Read more (PubMed: 26617239) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

We have not specifically tested ab138875 with tissue culture treated plates, but we do not anticipate any issues. Remember to use the top read mode on your plate reader for the assay.

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Answer

Thank you for your reply and for kindly confirming the details.

We have been provided the following sample preparation protocol from the labs:

Wash cells with cold PBS. Pellet 2X 105 cells for each assay in a micro-centrifuge tube (2000 rpm for 5 min). Extract the cells with 400 μl of PBS Buffer or buffer of your choice by freeze/thaw two cycles (20 min on dry-ice, then 10 min at room temperature), or homogenization. Votex the extraction for 10 sec. Spin the sample at 14000 rpm for 5 min. Transfer the extracted supernatant into a labeled tube or 96-well plate.


I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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