Renin Inhibitor Screening Assay Kit (Fluorometric) (ab204723)
- Datasheet
- References
- Protocols
Overview
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Product name
Renin Inhibitor Screening Assay Kit (Fluorometric)
See all Renin kits -
Detection method
Fluorescent -
Sample type
Inhibitor compounds -
Assay type
Enzyme activity -
Product overview
Renin Inhibitor Screening Assay Kit (Fluorometric) (ab204723) is an assay based on the use of a synthetic Renin peptide substrate with a fluorophore (EDANS) at one end and a quencher (DABCYL) at the other end. Renin catalyzes the cleavage of FRET substrate resulting in a product that is detected fluorometrically at Ex/Em = 328/552 nm. In the presence of a Renin inhibitor, the rate of hydrolysis of the substrate is decreased. The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Renin inhibitors also adaptable to a 384 well format.
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Notes
Renin (angiotensinogenase, EC 3.4.23.15) is an enzyme that participates in the renin-angiotensin system (RAS) which mediates extracellular volume (i.e. blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. An over-active renin-angiotensin system leads to vasoconstriction and retention of sodium and water, causing hypertension. Renin inhibitors are widely used for the treatment of hypertension.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Active Human Renin (lyophilized) 1 x 15µg Inhibitor Control [Aliskiren] (200 =µM) 1 x 10µl Renin Assay Buffer 1 x 25ml Renin Substrate 1 x 200µl -
Research areas
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Function
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. -
Involvement in disease
Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia. -
Sequence similarities
Belongs to the peptidase A1 family. -
Cellular localization
Secreted. Membrane. Associated to membranes via binding to ATP6AP2. - Information by UniProt
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Alternative names
- angiotensin-forming enzyme
- Angiotensinogenase
- HNFJ2
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Images
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Renin Inhibitor Screening Assay Kit (Fluorometric) (ab204723)Inhibition of Renin Enzyme Activity with Inhibitor Aliskiren.
Protocols
References
ab204723 has not yet been referenced specifically in any publications.
