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Renin Inhibitor Screening Assay Kit (Fluorometric) (ab204723)

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  • Protocol Booklet
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Renin Inhibitor Screening Assay Kit (Fluorometric) (ab204723)

    Key features and details

    • Assay type: Enzyme activity
    • Detection method: Fluorescent
    • Platform: Microplate reader
    • Sample type: Inhibitor compounds

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    Overview

    • Product name

      Renin Inhibitor Screening Assay Kit (Fluorometric)
      See all Renin kits
    • Detection method

      Fluorescent
    • Sample type

      Inhibitor compounds
    • Assay type

      Enzyme activity
    • Product overview

      Renin Inhibitor Screening Assay Kit (Fluorometric) (ab204723) is an assay based on the use of a synthetic Renin peptide substrate with a fluorophore (EDANS) at one end and a quencher (DABCYL) at the other end. Renin catalyzes the cleavage of FRET substrate resulting in a product that is detected fluorometrically at Ex/Em = 328/552 nm. In the presence of a Renin inhibitor, the rate of hydrolysis of the substrate is decreased. The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Renin inhibitors also adaptable to a 384 well format.

    • Notes

      Renin (angiotensinogenase, EC 3.4.23.15) is an enzyme that participates in the renin-angiotensin system (RAS) which mediates extracellular volume (i.e. blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. An over-active renin-angiotensin system leads to vasoconstriction and retention of sodium and water, causing hypertension. Renin inhibitors are widely used for the treatment of hypertension.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 100 tests
      Active Human Renin (lyophilized) 1 x 15µg
      Inhibitor Control [Aliskiren] (200 =µM) 1 x 10µl
      Renin Assay Buffer 1 x 25ml
      Renin Substrate 1 x 200µl
    • Research areas

      • Cardiovascular
      • Blood
      • Other
      • Signal Transduction
      • Metabolism
      • Vitamins / Minerals
      • Cardiovascular
      • Blood
      • Blood Pressure regulation
      • Kits/ Lysates/ Other
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      • Other Metabolism Assay
      • Metabolism
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      • Cofactors, Vitamins / minerals
      • Vitamins / minerals
    • Function

      Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
    • Involvement in disease

      Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
      Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia.
    • Sequence similarities

      Belongs to the peptidase A1 family.
    • Cellular localization

      Secreted. Membrane. Associated to membranes via binding to ATP6AP2.
    • Target information above from: UniProt accession P00797 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Angiotensin forming enzyme
      • Angiotensin forming enzyme precursor
      • Angiotensinogenase
      • Angiotensinogenase precursor
      • FLJ10761
      • HNFJ2
      • REN
      • Ren1
      • RENI_HUMAN
      • Renin
      • Renin precursor renal
      see all

    Associated products

      Images

      • Renin Inhibitor Screening Assay Kit (Fluorometric) (ab204723)
        Renin Inhibitor Screening Assay Kit (Fluorometric) (ab204723)

        Inhibition of Renin Enzyme Activity with Inhibitor Aliskiren.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab204723? Please let us know so that we can cite the reference in this datasheet.

      ab204723 has not yet been referenced specifically in any publications.

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