Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Product nameRenin Inhibitor Screening Assay Kit (Fluorometric)
See all Renin kits
Sample typeInhibitor compounds
Assay typeEnzyme activity
Renin Inhibitor Screening Assay Kit (Fluorometric) (ab204723) is an assay based on the use of a synthetic Renin peptide substrate with a fluorophore (EDANS) at one end and a quencher (DABCYL) at the other end. Renin catalyzes the cleavage of FRET substrate resulting in a product that is detected fluorometrically at Ex/Em = 328/552 nm. In the presence of a Renin inhibitor, the rate of hydrolysis of the substrate is decreased. The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Renin inhibitors also adaptable to a 384 well format.
Renin (angiotensinogenase, EC 126.96.36.199) is an enzyme that participates in the renin-angiotensin system (RAS) which mediates extracellular volume (i.e. blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. An over-active renin-angiotensin system leads to vasoconstriction and retention of sodium and water, causing hypertension. Renin inhibitors are widely used for the treatment of hypertension.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Active Human Renin (lyophilized) 1 x 15µg Inhibitor Control [Aliskiren] (200 =µM) 1 x 10µl Renin Assay Buffer 1 x 25ml Renin Substrate 1 x 200µl
FunctionRenin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Involvement in diseaseDefects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia.
Sequence similaritiesBelongs to the peptidase A1 family.
Cellular localizationSecreted. Membrane. Associated to membranes via binding to ATP6AP2.
- Information by UniProt
- Angiotensin forming enzyme
- Angiotensin forming enzyme precursor
ab204723 has not yet been referenced specifically in any publications.