Anti-Renin Receptor antibody (ab264763)
Key features and details
- Rabbit polyclonal to Renin Receptor
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Renin Receptor antibody
See all Renin Receptor primary antibodies -
Description
Rabbit polyclonal to Renin Receptor -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan -
Immunogen
Recombinant fragment corresponding to Human Renin Receptor aa 150 to the C-terminus.
Database link: O75787 -
Positive control
- WB: HEK-293 (human epithelial cell line from embryonic kidney) (transfected with control siRNA) cell lysate. IHC-P: Human pancreas, cerebellum, cerebral cortex, placenta and renal cell cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
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Related Buffer
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 39 kDa.
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Notes |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 39 kDa. |
Target
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Function
Functions as a renin and prorenin cellular receptor. May mediate renin-dependent cellular responses by activating ERK1 and ERK2. By increasing the catalytic efficiency of renin in AGT/angiotensinogen conversion to angiotensin I, it may also play a role in the renin-angiotensin system (RAS). -
Tissue specificity
Expressed in brain, heart, placenta, liver, kidney and pancreas. Barely detectable in lung and skeletal muscles. In the kidney cortex it is restricted to the mesangium of glomeruli. In the coronary and kidney artery it is expressed in the subendothelium, associated to smooth muscles where it colocalizes with REN. Expressed in vascular structures and by syncytiotrophoblast cells in the mature fetal placenta. -
Involvement in disease
Defects in ATP6AP2 are a cause of mental retardation X-linked with epilepsy (MRXE) [MIM:300423]. MRXE is a syndromic mental retardation. Patients manifest mild to moderate mental retardation associated with epilepsy, delays in motor milestones and speech acquisition in infancy. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 513520 Cow
- Entrez Gene: 10159 Human
- Entrez Gene: 70495 Mouse
- Entrez Gene: 302526 Rat
- Omim: 300556 Human
- SwissProt: P81134 Cow
- SwissProt: O75787 Human
- SwissProt: Q9CYN9 Mouse
see all -
Alternative names
- APT6M8 9 antibody
- APT6M8-9 antibody
- ATP6AP2 antibody
see all
Images
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All lanes : Anti-Renin Receptor antibody (ab264763) at 0.4 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) (transfected with control siRNA) cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) (transfected with target-specificl siRNA #1) cell lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) (transfected with target-specific siRNA #2) cell lysate
Predicted band size: 39 kDaLoading control: PPIB
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Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for Renin Receptor using ab264763 at 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human cerebellum tissue stained for Renin Receptor using ab264763 at 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human placenta tissue stained for Renin Receptor using ab264763 at 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded humanrenal cancer tissue stained for Renin Receptor using ab264763 at 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human pancreas tissue stained for Renin Receptor using ab264763 at 1/50 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab264763 has been referenced in 2 publications.
- Munro MJ et al. Colon adenocarcinoma-derived cells possessing stem cell function can be modulated using renin-angiotensin system inhibitors. PLoS One 16:e0256280 (2021). PubMed: 34428252
- Mohsin M et al. Increased (Pro)renin Receptor Expression in the Hypertensive Human Brain. Front Physiol 11:606811 (2020). PubMed: 33329061