Overview

  • Product name
  • Description
    Goat polyclonal to RENT1/hUPF1
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
  • Immunogen

    Immunogen was a synthetic peptide, to a region between residue 250 and 300 of human Regulator of Nonsense Transcripts 1 (up-frameshift mutation 1 homolog) using the numbering given in entry NP_002902.2 (GeneID 5976).).

  • Positive control
    • Whole cell lysate from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibodies were affinity purified using the peptide immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2500. Detects a band of approximately 140 kDa (predicted molecular weight: 134 kDa).

Target

  • Function
    Plays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the DNA2/NAM7 helicase family.
    Contains 1 C2H2-type zinc finger.
  • Domain
    The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
  • Post-translational
    modifications
    Phosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent helicase RENT1 antibody
    • ATP-dependent helicase RENT1 antibody
    • Delta helicase antibody
    • FLJ43809 antibody
    • FLJ46894 antibody
    • HUPF 1 antibody
    • hUpf1 antibody
    • KIAA0221 antibody
    • Nonsense mRNA reducing factor 1 antibody
    • NORF 1 antibody
    • NORF1 antibody
    • pNORF 1 antibody
    • pNORF1 antibody
    • Regulator of nonsense transcripts 1 antibody
    • RENT 1 antibody
    • RENT1 antibody
    • RENT1_HUMAN antibody
    • Smg 2 antibody
    • Smg 2 homolog nonsense mediated mRNA decay factor antibody
    • UP Frameshift 1 antibody
    • Up frameshift mutation 1 homolog (S. cerevisiae) antibody
    • Up frameshift mutation 1 homolog antibody
    • Up frameshift suppressor 1 homolog antibody
    • Up-frameshift suppressor 1 homolog antibody
    • UPF 1 antibody
    • UPF 1 regulator of nonsense transcripts homolog antibody
    • upf1 antibody
    • UPF1 regulator of nonsense transcripts homolog antibody
    • UPF1 RNA helicase and ATPase antibody
    • Yeast Upf1p homolog antibody
    see all

Images

  • ab10510 detection of human RENT1 by Western blot. Samples: Whole cell lysate (30 µg) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual).  Antibody: ab10510 used at 1 µg/ml. Detection: Chemiluminescence.

    ab10510 detection of human RENT1 by Western blot. Samples: Whole cell lysate (30 µg) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody: ab10510 used at 1 µg/ml. Detection: Chemiluminescence.

References

This product has been referenced in:
  • Truong DM  et al. Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation. PLoS Genet 11:e1005422 (2015). Read more (PubMed: 26241656) »
  • Henderson-Smith A  et al. SMG1 identified as a regulator of Parkinson's disease-associated alpha-synuclein through siRNA screening. PLoS One 8:e77711 (2013). WB . Read more (PubMed: 24204929) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Arabidopsis thaliana Tissue lysate - whole (10 day old seedlings, grown on MS medium)
Gel Running Conditions
Reduced Denaturing (8% gel)
Loading amount
100 µg
Specification
10 day old seedlings, grown on MS medium
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 22 2015

Answer

Thank you for contacting Abcam. Having looked at all our RENT1/UPF1 antibodies, I believe that ab10510 would be the most likely to work with your sample. Unfortunately I do not have access to the actual immunogen sequence that was used to generate the antibody. However as you mentioned in your original email, between residues 250-300, both Human and Arabidopsis share a high degree of homology and so there is a very good chance that antibody will react with Arabidopsis samples. As this antibody has not been tested in Arabidopsis, we cannot guarantee that it will work for you but what I can offer is our testing discount program. The details of which are below. Testing Discount Program As, ab10510 has not been tested in Arabidopsis, I can offer a discount off a future purchase if you buy ab10510 now, test it in Arabidopsis, and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab10510 in Arabidopsis. I will then send a discount code. This code must be issued before purchasing ab10510 so please wait for my reply before ordering. 2. Purchase ab10510 either by phone, fax, or online (www.abcam.com). 3. Test it in Arabidopsis. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab10510 turns out to be unsuitable for Arabidopsis, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us. We unfortunately do not have antibodies that are tested with Arabidopsis thaliana species. However after checking the immunogen sequence homology it seems that antibody ab10510 immunogen (amino acid 250 to 300) has about 83% sequence homology. So it is more likely that this antibody could detect the target protein in Arabidopsis thaliana. Arabidopsis thaliana is an untested species so this antibody is eligible for our Abreview testing discount code programme. The discount code will give you next primary antibody at no charge. It will be free. To activate the discount you have to provide us the tested Abreview. Let me know by email if you are interested the I will send you the discount code. I hope this will be helpful. Should you have any other question please do not hesitate to contact me.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Loading amount
50 µg
Specification
U2OS cells
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycine)
Blocking step
normal horse serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Natalie Farny

Verified customer

Submitted Oct 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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