Anti-RENT1/hUPF1 antibody (ab10510)
Key features and details
- Goat polyclonal to RENT1/hUPF1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RENT1/hUPF1 antibody
See all RENT1/hUPF1 primary antibodies -
Description
Goat polyclonal to RENT1/hUPF1 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus -
Immunogen
Synthetic peptide corresponding to Human RENT1/hUPF1 aa 250-350. NP_002902.2 (GeneID 5976)
Database link: Q92900 -
Positive control
- WB: Whole cell lysate from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual).; HeLa, NIH/3T3 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000 - 1/2500. Detects a band of approximately 140 kDa (predicted molecular weight: 134 kDa).
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Notes |
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WB
1/1000 - 1/2500. Detects a band of approximately 140 kDa (predicted molecular weight: 134 kDa). |
Target
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Function
Plays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the DNA2/NAM7 helicase family.
Contains 1 C2H2-type zinc finger. -
Domain
The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family. -
Post-translational
modificationsPhosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5976 Human
- Entrez Gene: 19704 Mouse
- Entrez Gene: 406783 Zebrafish
- Omim: 601430 Human
- SwissProt: Q92900 Human
- SwissProt: Q9EPU0 Mouse
- Unigene: 515266 Human
- Unigene: 390048 Mouse
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Alternative names
- ATP dependent helicase RENT1 antibody
- ATP-dependent helicase RENT1 antibody
- Delta helicase antibody
see all
Images
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All lanes : Anti-RENT1/hUPF1 antibody (ab10510) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate
Predicted band size: 134 kDa
Exposure time: 30 seconds -
ab10510 detection of human RENT1 by Western blot. Samples: Whole cell lysate (30
µ g) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody: ab10510 used at 1µ g/ml. Detection: Chemiluminescence.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab10510 has been referenced in 3 publications.
- Truong DM et al. Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation. PLoS Genet 11:e1005422 (2015). PubMed: 26241656
- Henderson-Smith A et al. SMG1 identified as a regulator of Parkinson's disease-associated alpha-synuclein through siRNA screening. PLoS One 8:e77711 (2013). WB . PubMed: 24204929
- Shi J et al. Caffeine regulates alternative splicing in a subset of cancer-associated genes: a role for SC35. Mol Cell Biol 28:883-95 (2008). WB ; Human . PubMed: 18025108