For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
To whom it may concern, I am interested in the RENT1/UPF1 antibodies that your company offers. Unfortunately, reading from the data sheets of the respective antibodies they are not supposed to react with the Arabidopsis thaliana homolog of UPF1. However, at least one of them was raised against a synthetic peptide between residues 250 and 300 from the human RENT1/UPF1, and this particular region is actually pretty well conserved. I understand that the peptide sequences are proprietary information but I would highly appreciate if you could compare the At sequence of UPF1 (which I have attached as a fasta file) with the antigens that you have used for your Antibodies and see whether they could crossreact? It would be really great to have a working antibody! Best regards,
Asked on Oct 11 2011
Thank you for contacting Abcam. Having looked at all our RENT1/UPF1 antibodies, I believe that ab10510 would be the most likely to work with your sample. Unfortunately I do not have access to the actual immunogen sequence that was used to generate the antibody. However as you mentioned in your original email, between residues 250-300, both Human and Arabidopsis share a high degree of homology and so there is a very good chance that antibody will react with Arabidopsis samples. As this antibody has not been tested in Arabidopsis, we cannot guarantee that it will work for you but what I can offer is our testing discount program. The details of which are below. Testing Discount Program As, ab10510 has not been tested in Arabidopsis, I can offer a discount off a future purchase if you buy ab10510 now, test it in Arabidopsis, and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab10510 in Arabidopsis. I will then send a discount code. This code must be issued before purchasing ab10510 so please wait for my reply before ordering. 2. Purchase ab10510 either by phone, fax, or online (www.abcam.com). 3. Test it in Arabidopsis. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab10510 turns out to be unsuitable for Arabidopsis, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.
Answered on Oct 11 2011