Overview

  • Product name

  • Description

    Goat polyclonal to RENT1/hUPF1
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of the C-terminus of human regulator of nonsense transcripts 1 (RENT1)(LocusLink ID 5976).

  • Positive control

    • Whole cell lysate from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibodies were affinity purified using the peptide immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2500. Detects a band of approximately 135 kDa (predicted molecular weight: 134 kDa).

Target

  • Function

    Plays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the DNA2/NAM7 helicase family.
    Contains 1 C2H2-type zinc finger.
  • Domain

    The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
  • Post-translational
    modifications

    Phosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP dependent helicase RENT1 antibody
    • ATP-dependent helicase RENT1 antibody
    • Delta helicase antibody
    • FLJ43809 antibody
    • FLJ46894 antibody
    • HUPF 1 antibody
    • hUpf1 antibody
    • KIAA0221 antibody
    • Nonsense mRNA reducing factor 1 antibody
    • NORF 1 antibody
    • NORF1 antibody
    • pNORF 1 antibody
    • pNORF1 antibody
    • Regulator of nonsense transcripts 1 antibody
    • RENT 1 antibody
    • RENT1 antibody
    • RENT1_HUMAN antibody
    • Smg 2 antibody
    • Smg 2 homolog nonsense mediated mRNA decay factor antibody
    • UP Frameshift 1 antibody
    • Up frameshift mutation 1 homolog (S. cerevisiae) antibody
    • Up frameshift mutation 1 homolog antibody
    • Up frameshift suppressor 1 homolog antibody
    • Up-frameshift suppressor 1 homolog antibody
    • UPF 1 antibody
    • UPF 1 regulator of nonsense transcripts homolog antibody
    • upf1 antibody
    • UPF1 regulator of nonsense transcripts homolog antibody
    • UPF1 RNA helicase and ATPase antibody
    • Yeast Upf1p homolog antibody
    see all

Images

  • ab10534 Detection of Human RENT1 by Western blot. Samples: Whole cell lysate (30 mg) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody: ab10534 used at 1 ug/ml. Detection: Chemiluminescence.

    ab10534 Detection of Human RENT1 by Western blot. Samples: Whole cell lysate (30 mg) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody: ab10534 used at 1 ug/ml. Detection: Chemiluminescence.

References

This product has been referenced in:

  • Zhao C  et al. Stress-sensitive regulation of IFRD1 mRNA decay is mediated by an upstream open reading frame. J Biol Chem 285:8552-62 (2010). WB ; Human . Read more (PubMed: 20080976) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us. While we do carry a number of anti-hUPF1 antibodies which have been generated using immunogens near the C-terminus, the epitope for ab109363 is generated from a region near the N-terminus. A number of our other anti-hUPF1 antibodies which have been created from the C-terminus region have their immunogen sequence listed. The exception is ab10534. This product’s epitope maps to a region near the C-terminus of human Regulator of Nonsense Transcripts 1 (up-frame shift mutation 1 homolog) using the numbering given in entry NP_002902.2 (GeneID 5976). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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Answer

Thank you for contacting Abcam about ab109363, ab92655 and ab10534. Unfortunately the immunogen sequences for these three antibodies is proprietary information and I do not have access to that information. Please except my apologies for not being to provide you with the information requested. If there is anything else that I can help you with, please do not hesitate to contact me.

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