Anti-RENT1/hUPF1 antibody - N-terminal (ab154903)
Key features and details
- Rabbit polyclonal to RENT1/hUPF1 - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RENT1/hUPF1 antibody - N-terminal
See all RENT1/hUPF1 primary antibodies -
Description
Rabbit polyclonal to RENT1/hUPF1 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment, corresponding to a region within N-terminal amino acids 95-352 of Human RENT1/hUPF1 (Uniprot ID: Q92900).
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Positive control
- A431 whole cell lysate; Human colon carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Zebrafish
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 124 kDa.
|
|
| IHC-P |
1/100 - 1/1000.
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| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 124 kDa. |
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IHC-P
1/100 - 1/1000. |
Target
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Function
Plays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the DNA2/NAM7 helicase family.
Contains 1 C2H2-type zinc finger. -
Domain
The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family. -
Post-translational
modificationsPhosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5976 Human
- Entrez Gene: 19704 Mouse
- Entrez Gene: 406783 Zebrafish
- Omim: 601430 Human
- SwissProt: Q92900 Human
- SwissProt: Q9EPU0 Mouse
- Unigene: 515266 Human
- Unigene: 390048 Mouse
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Alternative names
- ATP dependent helicase RENT1 antibody
- ATP-dependent helicase RENT1 antibody
- Delta helicase antibody
see all
Images
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Western blot - Anti-RENT1/hUPF1 antibody - N-terminal (ab154903)Anti-RENT1/hUPF1 antibody - N-terminal (ab154903) at 1/500 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 124 kDa
5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RENT1/hUPF1 antibody - N-terminal (ab154903)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling RENT1/hUPF1 with ab154903 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154903 has not yet been referenced specifically in any publications.