Anti-RENT1/hUPF1 antibody - N-terminal (ab154903)
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Overview
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Product name
Anti-RENT1/hUPF1 antibody - N-terminal
See all RENT1/hUPF1 primary antibodies -
Description
Rabbit polyclonal to RENT1/hUPF1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Zebrafish
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Immunogen
Recombinant fragment, corresponding to a region within N-terminal amino acids 95-352 of Human RENT1/hUPF1 (Uniprot ID: Q92900).
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Positive control
- A431 whole cell lysate; Human colon carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 1% BSA, 78% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 124 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
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Function
Plays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the DNA2/NAM7 helicase family.
Contains 1 C2H2-type zinc finger. -
Domain
The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family. -
Post-translational
modificationsPhosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5976 Human
- Entrez Gene: 19704 Mouse
- Entrez Gene: 406783 Zebrafish
- Omim: 601430 Human
- SwissProt: Q92900 Human
- SwissProt: Q9EPU0 Mouse
- Unigene: 515266 Human
- Unigene: 390048 Mouse
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Alternative names
- ATP dependent helicase RENT1 antibody
- ATP-dependent helicase RENT1 antibody
- Delta helicase antibody
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Images
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Western blot - Anti-RENT1/hUPF1 antibody - N-terminal (ab154903)Anti-RENT1/hUPF1 antibody - N-terminal (ab154903) at 1/500 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 124 kDa
5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RENT1/hUPF1 antibody - N-terminal (ab154903)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling RENT1/hUPF1 with ab154903 at 1/500 dilution.
References
ab154903 has not yet been referenced specifically in any publications.
