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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Other
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Anti-RENT1/hUPF1 antibody - N-terminal (ab154903)

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Western blot - Anti-RENT1/hUPF1 antibody - N-terminal (ab154903)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RENT1/hUPF1 antibody - N-terminal (ab154903)

Key features and details

  • Rabbit polyclonal to RENT1/hUPF1 - N-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-RENT1/hUPF1 antibody - N-terminal
    See all RENT1/hUPF1 primary antibodies
  • Description

    Rabbit polyclonal to RENT1/hUPF1 - N-terminal
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to a region within N-terminal amino acids 95-352 of Human RENT1/hUPF1 (Uniprot ID: Q92900).

  • Positive control

    • A431 whole cell lysate; Human colon carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A-431 whole cell lysate (ab7909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Zebrafish
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 124 kDa.
IHC-P
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 124 kDa.
IHC-P
1/100 - 1/1000.

Target

  • Function

    Plays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the DNA2/NAM7 helicase family.
    Contains 1 C2H2-type zinc finger.
  • Domain

    The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
  • Post-translational
    modifications

    Phosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm.
  • Target information above from: UniProt accession Q92900 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5976 Human
    • Entrez Gene: 19704 Mouse
    • Entrez Gene: 406783 Zebrafish
    • Omim: 601430 Human
    • SwissProt: Q92900 Human
    • SwissProt: Q9EPU0 Mouse
    • Unigene: 515266 Human
    • Unigene: 390048 Mouse
    • Alternative names

      • ATP dependent helicase RENT1 antibody
      • ATP-dependent helicase RENT1 antibody
      • Delta helicase antibody
      • FLJ43809 antibody
      • FLJ46894 antibody
      • HUPF 1 antibody
      • hUpf1 antibody
      • KIAA0221 antibody
      • Nonsense mRNA reducing factor 1 antibody
      • NORF 1 antibody
      • NORF1 antibody
      • pNORF 1 antibody
      • pNORF1 antibody
      • Regulator of nonsense transcripts 1 antibody
      • RENT 1 antibody
      • RENT1 antibody
      • RENT1_HUMAN antibody
      • Smg 2 antibody
      • Smg 2 homolog nonsense mediated mRNA decay factor antibody
      • UP Frameshift 1 antibody
      • Up frameshift mutation 1 homolog (S. cerevisiae) antibody
      • Up frameshift mutation 1 homolog antibody
      • Up frameshift suppressor 1 homolog antibody
      • Up-frameshift suppressor 1 homolog antibody
      • UPF 1 antibody
      • UPF 1 regulator of nonsense transcripts homolog antibody
      • upf1 antibody
      • UPF1 regulator of nonsense transcripts homolog antibody
      • UPF1 RNA helicase and ATPase antibody
      • Yeast Upf1p homolog antibody
      see all

    Images

    • Western blot - Anti-RENT1/hUPF1 antibody - N-terminal (ab154903)
      Western blot - Anti-RENT1/hUPF1 antibody - N-terminal (ab154903)
      Anti-RENT1/hUPF1 antibody - N-terminal (ab154903) at 1/500 dilution + A431 whole cell lysate at 30 µg

      Predicted band size: 124 kDa



      5% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RENT1/hUPF1 antibody - N-terminal (ab154903)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RENT1/hUPF1 antibody - N-terminal (ab154903)
      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling RENT1/hUPF1 with ab154903 at 1/500 dilution.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab154903? Please let us know so that we can cite the reference in this datasheet.

    ab154903 has not yet been referenced specifically in any publications.

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