Recombinant Anti-REPS2 antibody [EPR12033(B)] (ab170883)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12033(B)] to REPS2
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-REPS2 antibody [EPR12033(B)]
See all REPS2 primary antibodies -
Description
Rabbit monoclonal [EPR12033(B)] to REPS2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human REPS2 aa 550-650 (Cysteine residue). The exact sequence is proprietary.
Database link: Q8NFH8 -
Positive control
- LnCap, T47-D and 293T cell lysates
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12033(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab170883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/10000 - 1/50000. Predicted molecular weight: 72 kDa. | |
IP | 1/10 - 1/100. |
Target
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Relevance
REPS2 is part of a protein complex that regulates the endocytosis of growth factor receptors. It directly interacts with a GTPase activating protein that functions downstream of the small G protein Ral. Its expression can negatively affect receptor internalization and inhibit growth factor signaling. Multiple transcript variants encoding different isoforms have been found for this gene. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 9185 Human
- Omim: 300317 Human
- SwissProt: Q8NFH8 Human
- Unigene: 186810 Human
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Alternative names
- OTTHUMP00000022995 antibody
- Partner of Ral binding protein 1 antibody
- Partner of RalBP1 antibody
see all
Images
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Western blot analysis on immunoprecipitation pellet from 293T cell lysate labeling REPS2 using ab170883 at a 1/10 dilution.
Anti-REPS2 antibody [EPR12033(B)] (ab170883) at 1/10 dilution + pellet from 293T cell lysate -
All lanes : Anti-REPS2 antibody [EPR12033(B)] (ab170883) at 1/10000 dilution
Lane 1 : LnCap lysate
Lane 2 : T47-D lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 72 kDa
Protocols
Datasheets and documents
References (0)
ab170883 has not yet been referenced specifically in any publications.