Anti-REPS2 antibody [EPR12033(B)] (ab170883)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-REPS2 antibody [EPR12033(B)]
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DescriptionRabbit monoclonal [EPR12033(B)] to REPS2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human REPS2 aa 550-650 (Cysteine residue). The exact sequence is proprietary.
Database link: Q8NFH8 -
Positive control
- LnCap, T47-D and 293T cell lysates
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR12033(B)
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab170883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 72 kDa. | |
| IP | 1/10 - 1/100. |
Target
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RelevanceREPS2 is part of a protein complex that regulates the endocytosis of growth factor receptors. It directly interacts with a GTPase activating protein that functions downstream of the small G protein Ral. Its expression can negatively affect receptor internalization and inhibit growth factor signaling. Multiple transcript variants encoding different isoforms have been found for this gene.
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Cellular localizationCytoplasmic
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Database links
- Entrez Gene: 9185 Human
- Omim: 300317 Human
- SwissProt: Q8NFH8 Human
- Unigene: 186810 Human
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Alternative names
- OTTHUMP00000022995 antibody
- Partner of Ral binding protein 1 antibody
- Partner of RalBP1 antibody
see all
Images
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![Immunoprecipitation - Anti-REPS2 antibody [EPR12033(B)] (ab170883)](https://www.abcam.com/ps/products/170/ab170883/Images/ab170883-171433-ab170883-2.jpg)
Immunoprecipitation - Anti-REPS2 antibody [EPR12033(B)] (ab170883)Western blot analysis on immunoprecipitation pellet from 293T cell lysate labeling REPS2 using ab170883 at a 1/10 dilution.
Anti-REPS2 antibody [EPR12033(B)] (ab170883) at 1/10 dilution + pellet from 293T cell lysate -
![Western blot - Anti-REPS2 antibody [EPR12033(B)] (ab170883)](https://www.abcam.com/ps/products/170/ab170883/Images/ab170883-171432-ab170883.jpg)
Western blot - Anti-REPS2 antibody [EPR12033(B)] (ab170883)All lanes : Anti-REPS2 antibody [EPR12033(B)] (ab170883) at 1/10000 dilution
Lane 1 : LnCap lysate
Lane 2 : T47-D lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 72 kDa
References
ab170883 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"