Overview

  • Product name

    Anti-Reptin/TIP49B/RUVB2 antibody
    See all Reptin/TIP49B/RUVB2 primary antibodies
  • Description

    Rabbit polyclonal to Reptin/TIP49B/RUVB2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-303 of Human Reptin/TIP49B/RUVB2 (UniProt ID: Q9Y230).

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, Molt4 and Raji whole cell lysates; A549 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab137834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 51 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.
  • Tissue specificity

    Ubiquitously expressed. Highly expressed in testis and thymus.
  • Sequence similarities

    Belongs to the ruvB family.
  • Domain

    The C-terminal domain is required for association with ATF2.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • 48 kDa TATA box-binding protein-interacting protein antibody
    • 48 kDa TBP-interacting protein antibody
    • 48-kDa TATA box-binding protein-interacting protein antibody
    • 48-kDa TBP-interacting protein antibody
    • 51 kDa erythrocyte cytosolic protein antibody
    • CGI-46 antibody
    • EC=3.6.1.- antibody
    • ECP-51 antibody
    • ECP51 antibody
    • Erythrocyte cytosolic protein, 51-KD antibody
    • INO80 complex subunit J antibody
    • INO80J antibody
    • MGC144733 antibody
    • MGC144734 antibody
    • MGC52995 antibody
    • mp47 antibody
    • p47 antibody
    • p47 protein antibody
    • Repressing pontin 52 antibody
    • Reptin 52 antibody
    • REPTIN antibody
    • RuvB (E coli homolog)-like 2 antibody
    • RUVB, E. coli, homolog-like 2 antibody
    • RuvB-like 2 (E. coli) antibody
    • RuvB-like 2 antibody
    • RuvB-like protein 2 antibody
    • RUVB2 antibody
    • RUVB2_HUMAN antibody
    • RUVBL2 antibody
    • RVB2 antibody
    • TAP54-beta antibody
    • TATA box-binding protein-interacting protein, 48-KD antibody
    • TBP-interacting protein, 48-KD antibody
    • TIH2 antibody
    • TIP48 antibody
    • TIP49b antibody
    • TIP60-associated protein 54-beta antibody
    • wu:fi25f01 antibody
    • zreptin antibody
    see all

Images

  • Anti-Reptin/TIP49B/RUVB2 antibody (ab137834) at 1/1000 dilution + H2199 whole cell lysate at 30 µg

    Predicted band size: 51 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde fixed A549 cells, labeling RUVBL2 using ab137834 at a 1/200 dilution.
    The lower image is merged with a DNA probe.

References

This product has been referenced in:

  • Raymond AA  et al. Reptin regulates DNA double strand breaks repair in human hepatocellular carcinoma. PLoS One 10:e0123333 (2015). WB ; Human . Read more (PubMed: 25875766) »
See 1 Publication for this product

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