Overview

  • Product name

    Anti-Reptin/TIP49B/RUVB2 antibody [EP4145(2)]
    See all Reptin/TIP49B/RUVB2 primary antibodies
  • Description

    Rabbit monoclonal [EP4145(2)] to Reptin/TIP49B/RUVB2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Reptin/TIP49B/RUVB2 aa 50-150. The exact sequence is proprietary.
    Database link: Q9Y230

  • Positive control

    • HepG2, Daudi, HeLa, SK-BR-3, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab196027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).

Target

  • Function

    Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.
  • Tissue specificity

    Ubiquitously expressed. Highly expressed in testis and thymus.
  • Sequence similarities

    Belongs to the ruvB family.
  • Domain

    The C-terminal domain is required for association with ATF2.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • 48 kDa TATA box-binding protein-interacting protein antibody
    • 48 kDa TBP-interacting protein antibody
    • 48-kDa TATA box-binding protein-interacting protein antibody
    • 48-kDa TBP-interacting protein antibody
    • 51 kDa erythrocyte cytosolic protein antibody
    • CGI-46 antibody
    • EC=3.6.1.- antibody
    • ECP-51 antibody
    • ECP51 antibody
    • Erythrocyte cytosolic protein, 51-KD antibody
    • INO80 complex subunit J antibody
    • INO80J antibody
    • MGC144733 antibody
    • MGC144734 antibody
    • MGC52995 antibody
    • mp47 antibody
    • p47 antibody
    • p47 protein antibody
    • Repressing pontin 52 antibody
    • Reptin 52 antibody
    • REPTIN antibody
    • RuvB (E coli homolog)-like 2 antibody
    • RUVB, E. coli, homolog-like 2 antibody
    • RuvB-like 2 (E. coli) antibody
    • RuvB-like 2 antibody
    • RuvB-like protein 2 antibody
    • RUVB2 antibody
    • RUVB2_HUMAN antibody
    • RUVBL2 antibody
    • RVB2 antibody
    • TAP54-beta antibody
    • TATA box-binding protein-interacting protein, 48-KD antibody
    • TBP-interacting protein, 48-KD antibody
    • TIH2 antibody
    • TIP48 antibody
    • TIP49b antibody
    • TIP60-associated protein 54-beta antibody
    • wu:fi25f01 antibody
    • zreptin antibody
    see all

Images

  • All lanes : Anti-Reptin/TIP49B/RUVB2 antibody [EP4145(2)] (ab196027) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 51 kDa

  • All lanes : Anti-Reptin/TIP49B/RUVB2 antibody [EP4145(2)] (ab196027) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Daudi cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : SK-BR-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 51 kDa

References

ab196027 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
U2OS
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jul 25 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up