Anti-Reptin/TIP49B/RUVB2 antibody [EP4145(2)] (ab196027)
Key features and details
- Rabbit monoclonal [EP4145(2)] to Reptin/TIP49B/RUVB2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Related conjugates and formulations
Overview
-
Product name
Anti-Reptin/TIP49B/RUVB2 antibody [EP4145(2)]
See all Reptin/TIP49B/RUVB2 primary antibodies -
Description
Rabbit monoclonal [EP4145(2)] to Reptin/TIP49B/RUVB2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HepG2, Daudi, HeLa, SK-BR-3, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP4145(2) -
Isotype
IgG -
Research areas
Associated products
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).
|
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa). |
Target
-
Function
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2. -
Tissue specificity
Ubiquitously expressed. Highly expressed in testis and thymus. -
Sequence similarities
Belongs to the ruvB family. -
Domain
The C-terminal domain is required for association with ATF2. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes. - Information by UniProt
-
Database links
- Entrez Gene: 10856 Human
- Entrez Gene: 20174 Mouse
- Entrez Gene: 292907 Rat
- Omim: 604788 Human
- SwissProt: Q9Y230 Human
- SwissProt: Q9WTM5 Mouse
- Unigene: 515846 Human
- Unigene: 34410 Mouse
-
Alternative names
- 48 kDa TATA box-binding protein-interacting protein antibody
- 48 kDa TBP-interacting protein antibody
- 48-kDa TATA box-binding protein-interacting protein antibody
see all
Images
-
All lanes : Anti-Reptin/TIP49B/RUVB2 antibody [EP4145(2)] (ab196027) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 51 kDa -
All lanes : Anti-Reptin/TIP49B/RUVB2 antibody [EP4145(2)] (ab196027) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : SK-BR-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 51 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab196027 has not yet been referenced specifically in any publications.