Key features and details
- Rabbit polyclonal to Repulsive Guidance Molecule A
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Repulsive Guidance Molecule A antibody
See all Repulsive Guidance Molecule A primary antibodies
DescriptionRabbit polyclonal to Repulsive Guidance Molecule A
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse
Predicted to work with: Human
- IHC-P: Mouse embryo tissue. WB: Mouse cerebellum
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab216643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 49 kDa.|
|IHC-P||1/100 - 1/500.|
FunctionMember of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.
Sequence similaritiesBelongs to the repulsive guidance molecule (RGM) family.
Cellular localizationCell membrane.
- Information by UniProt
- Repulsive guidance molecule A antibody
- RGM antibody
- RGM domain family member A antibody
Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse embryo tissue labeling Repulsive Guidance Molecule A using ab216643 at 1/200, followed by secondary detection and DAB staining.
Anti-Repulsive Guidance Molecule A antibody (ab216643) at 1/500 dilution + Mouse cerebellum tissue lysate
Predicted band size: 49 kDa
ab216643 has been referenced in 1 publication.
- Zhang Y et al. Changes of fractional anisotropy and RGMa in crossed cerebellar diaschisis induced by middle cerebral artery occlusion. Exp Ther Med 18:3595-3602 (2019). PubMed: 31602236