Overview

  • Product name

    Anti-Repulsive Guidance Molecule A antibody [EP7990(2)]
    See all Repulsive Guidance Molecule A primary antibodies
  • Description

    Rabbit monoclonal [EP7990(2)] to Repulsive Guidance Molecule A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Repulsive Guidance Molecule A aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q96B86

  • Positive control

    • Fetal brain, A673 and HeLa lysates, HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 49 kDa).
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.
    • Sequence similarities

      Belongs to the repulsive guidance molecule (RGM) family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Repulsive guidance molecule A antibody
      • RGM antibody
      • RGM domain family member A antibody
      • RGMA antibody
      • RGMA_HUMAN antibody
      see all

    Images

    • Immunofluorescent analysis of HeLa cells labeling Repulsive Guidance Molecule A with ab169761 at 1/50 (red) and DAPI nuclear
      staining (blue).

    • All lanes : Anti-Repulsive Guidance Molecule A antibody [EP7990(2)] (ab169761) at 1/10000 dilution

      Lane 1 : fetal brain lysate
      Lane 2 : A673 lysate
      Lane 3 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 49 kDa
      Additional bands at: 37 kDa (possible mature (processed) protein)

    References

    This product has been referenced in:

    • Zhang R  et al. RGMa mediates reactive astrogliosis and glial scar formation through TGFß1/Smad2/3 signaling after stroke. Cell Death Differ 25:1503-1516 (2018). Read more (PubMed: 29396549) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab169761.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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